Method for high-sensitivity and low-background detection of fumonisin B1 through hydrogel silica photon crystal microsphere chemiluminescence method

Abstract

The present invention relates to a method for high-sensitivity detection of fumonisin B1 through a hydrogel silica photon crystal microsphere chemiluminescence method. According to the present invention, the gel is subjected to ultraviolet polymerization in the silica photon crystal microsphere gap so as to reduce the porous structure inside the microsphere, reduce the non-specific biological reaction background signal, and improve the detection sensitivity and the detection accuracy; and the fumonisin B1 probe carrier on the microsphere surface is used to construct the competitive fumonisin B1 chemiluminescent immunodetection system on the microsphere surface, the chemiluminescent signal is detected by using the multifunctional enzyme-labeling measuring instrument, the detection limit of the method on the fumonisin B1 is 0.0001 ng / mL, the linear detection range is 0.0001-1 ng / mL, and the method has advantages of high detection sensitivity and low background signal.

Description

technical field [0001] The invention designs a technical method for detecting fumonisins in agricultural products, feed and food with high sensitivity, low background and low cost. Specifically, hydrogel silica photonic crystal microspheres were synthesized by self-assembly technology and ultraviolet polymerization technology, and the photonic crystal was used as a carrier to build a competitive immunochemiluminescent, high-sensitivity, low-background, low-cost detection voltage on the surface of the microspheres. New approach to equine toxin. Background technique [0002] Fumonisins are metabolites produced by fungi of the genus Fusariumgenus. Fumonisin contamination is widespread, especially in corn and its products. Fumonisins mainly affect the liver and kidneys of animals. Long-term or high-dose exposure can induce liver cancer, and also cause varying degrees of damage to embryos, lungs, musculoskeletal, etc., especially fumonisin B1 (FB1), which can make humans and an...

AI Technical Summary

Problems solved by technology

However, the chromatographic method requires expensive detection equipment and specialized instrument operation technicians, and the detection cost is high, so it is difficult to promote and popularize in the front line of food safety production and sales. The detection sensitivity is not enough, and the detection needs to consume a large amount of antibody reagents; the detection of mycotoxins based on porous surface immunoassays such as photonic crystal microsphere liquid chip chemiluminescence method, due to its high detection background signal, limited detection limit, and poor accuracy

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