Method for high-sensitivity and low-background detection of fumonisin B1 through hydrogel silica photon crystal microsphere chemiluminescence method

A technology of photonic crystal microspheres and chemiluminescence, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of high detection background signal, high detection cost, and large consumption of antibody reagents, so as to expand the detection linear range and reduce Detect background signal, save reagent and sample effect

Inactive Publication Date: 2015-12-16
NANJING NORMAL UNIVERSITY
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Problems solved by technology

However, the chromatographic method requires expensive detection equipment and specialized instrument operation technicians, and the detection cost is high, so it is difficult to promote and popularize in the front line of food safety production and sales. The detection sensitivity is not enoug...

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  • Method for high-sensitivity and low-background detection of fumonisin B1 through hydrogel silica photon crystal microsphere chemiluminescence method
  • Method for high-sensitivity and low-background detection of fumonisin B1 through hydrogel silica photon crystal microsphere chemiluminescence method
  • Method for high-sensitivity and low-background detection of fumonisin B1 through hydrogel silica photon crystal microsphere chemiluminescence method

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Embodiment 1

[0038] Rice, corn and wheat are pulverized with a traditional Chinese medicine grinder, passed through a 20-mesh sieve, weighed 5g of each sample in a 100mL conical flask, added fumonisin B1 standard substances of different concentrations and volumes to the sample, and mixed the sample thoroughly , put it in a ventilated place until the solvent evaporates completely. Add 15mL of extractant (methanol:water=6:4) to it, extract in a shaker at 150r / min for 2 hours, filter with whatman filter paper, and the filtrate is the sample to be tested. At the same time, extract the control sample without adding standard substance according to the above method.

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Abstract

The present invention relates to a method for high-sensitivity detection of fumonisin B1 through a hydrogel silica photon crystal microsphere chemiluminescence method. According to the present invention, the gel is subjected to ultraviolet polymerization in the silica photon crystal microsphere gap so as to reduce the porous structure inside the microsphere, reduce the non-specific biological reaction background signal, and improve the detection sensitivity and the detection accuracy; and the fumonisin B1 probe carrier on the microsphere surface is used to construct the competitive fumonisin B1 chemiluminescent immunodetection system on the microsphere surface, the chemiluminescent signal is detected by using the multifunctional enzyme-labeling measuring instrument, the detection limit of the method on the fumonisin B1 is 0.0001 ng/mL, the linear detection range is 0.0001-1 ng/mL, and the method has advantages of high detection sensitivity and low background signal.

Description

technical field [0001] The invention designs a technical method for detecting fumonisins in agricultural products, feed and food with high sensitivity, low background and low cost. Specifically, hydrogel silica photonic crystal microspheres were synthesized by self-assembly technology and ultraviolet polymerization technology, and the photonic crystal was used as a carrier to build a competitive immunochemiluminescent, high-sensitivity, low-background, low-cost detection voltage on the surface of the microspheres. New approach to equine toxin. Background technique [0002] Fumonisins are metabolites produced by fungi of the genus Fusariumgenus. Fumonisin contamination is widespread, especially in corn and its products. Fumonisins mainly affect the liver and kidneys of animals. Long-term or high-dose exposure can induce liver cancer, and also cause varying degrees of damage to embryos, lungs, musculoskeletal, etc., especially fumonisin B1 (FB1), which can make humans and an...

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 李建林徐杰李玮杨浥徐婧婧沈鹏刘蕊郑铁松
Owner NANJING NORMAL UNIVERSITY
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