Compound for restraining phomopsis helianthi growth

The technology of stem canker and sunflower is applied in the field of bacteriostatic and bactericidal activities of sunflower stem canker, and can solve the problems of disease resistance, lodging resistance, yield and quality decline, and high empty shell rate of varieties.

Inactive Publication Date: 2015-12-16
许自协
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term planting of a single variety will result in reduced disease resistance and lodging resistance of the variety, and a high empty shell rate, resulting in a decline in yield and quality.

Method used

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  • Compound for restraining phomopsis helianthi growth
  • Compound for restraining phomopsis helianthi growth

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Measure the minimum inhibitory concentration MIC value.

[0017] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0018] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0019] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0020] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially on...

Embodiment 2

[0027] Measure the minimum bactericidal concentration MBC value.

[0028] (1) Preparation of nutrient broth: Take 30g of nutrient broth and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0029] (2) Preparation of nutrient agar solid medium: take 45g of nutrient agar and add 1000mL of distilled water. Sterilize by high pressure steam at 121°C for 20 minutes before use.

[0030] (3) Cultivation of bacterial strains: the operation is carried out on a clean bench. Draw 10mL of sterilized liquid culture medium, put it in a sterilized test tube, then pick a colony with the inoculation loop, add it to the liquid medium, and put it in the incubator for cultivation. The bacteria are cultivated for 24 hours at a temperature of 28°C.

[0031] (4) Bacterial solution preparation and counting: Dilute the cultured bacterial solution with liquid medium by 10-fold dilution method, and use a hemocytometer to observe and count initially ...

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Abstract

The invention discloses a compound which has inhibitory and bactericidal activity for phomopsis helianthi. The minimum inhibitory concentration (MIC) value of phomopsis helianthi is 0.5 microgram/mL, and the minimum bactericidal concentration (MBC) value of phomopsis helianthi is 0.2 microgram/mL.

Description

technical field [0001] The invention relates to the new application of the compound, clarifies the relationship between the chemical structure of the medicine and the biological activity of the bacteria, more specifically, explores the antibacterial and bactericidal activities of the compound on the sunflower stem canker bacterium. Background technique [0002] Sunflower is native to the Americas. Its English name is sunflower. It is a plant of Compositae. Its wild species are mainly distributed in the south and west of North America between 30° and 52° in North Korea, as well as in Peru and northern Mexico. The wild crops are domesticated by local people for agricultural crops. Sunflower is divided into three types: oil-use type, practical type and intermediate type. The oil-use type is oil sunflower, which has become the second largest edible oil crop in the world after soybean. The oil content of sunflower seeds is generally above 45%. Internationally, sunflower is recog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D413/04A01P3/00
CPCC07D413/04
Inventor 不公告发明人
Owner 许自协
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