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Lactobacillus plantarum grx16 and its application

A technology of Lactobacillus plantarum, CGMCCNO.10921, applied in application, bacteria, microorganisms, etc., can solve the problems of drug treatment limitations, blurred vision, muscle atrophy, etc., achieve excellent in vitro inhibition of α-glucosidase activity, and have a good market Prospect, effect of strong reducing activity

Active Publication Date: 2018-07-06
上海昊岳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs and insulin replacement have been widely used in the treatment of diabetes, but the side effects of drugs may lead to other symptoms such as physical discomfort, even direct use of insulin injections can also cause muscle atrophy at the injection site, blurred vision and other side effects, which makes drug treatment in Clinical applications are largely limited

Method used

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  • Lactobacillus plantarum grx16 and its application
  • Lactobacillus plantarum grx16 and its application
  • Lactobacillus plantarum grx16 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Isolation, screening method and identification of bacterial strains.

[0014] 1. Sample collection

[0015] The samples of long-lived people and their families in Bama Changshou Village, Guangxi were collected, sealed with 1 mL of sterilized liquid paraffin oil, and quickly placed in an ice box to separate the lactic acid bacteria in the samples.

[0016] 2. Isolation of lactic acid bacteria

[0017] After the collected samples were serially diluted, they were respectively inoculated in MRS solid medium, LBS solid medium and PTYG solid medium, and cultured anaerobically at 37°C for 48 hours. Typical colonies on the plate were picked and separated by streaking to obtain pure colonies. The pure colonies on each plate were picked and placed in MRS liquid medium, and after anaerobic culture at 37°C for 24 hours, they were refrigerated at 4°C for standby or freeze-dried for storage.

[0018] 3. Physiological and biochemical tests of lactic acid bacteria

[0...

Embodiment 2

[0079] Embodiment 2. The production method of fermented milk containing the strain of the present invention After the raw milk is standardized, add 6% sugar, preheat to 60-65°C, homogenize at 12-20Mpa pressure, heat treat at 95°C / 5min, and cool to 42 -45°C, inoculate 4 strains for mixed fermentation according to 3% inoculation amount, ferment at 37°C until curdling, and then store at 4°C.

Embodiment 3

[0080] Example 3. The production method of fermented camel milk containing the strain of the present invention. The raw milk is standardized and homogenized at 2.20Mpa pressure. After heat treatment at 65°C / 30min, it is cooled to 42-45°C and mixed with 4 strains inoculated with 4% inoculation amount. Fermentation, ferment at 37°C to pH 4.4-4.6, then store at 4°C.

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Abstract

The invention relates to the isolation, screening and identification of lactic acid bacteria capable of inhibiting the activity of α-glucosidase, in particular to a plant lactobacillus and its application. Lactic acid bacteria were isolated from the intestines of long-lived people in Bama, Guangxi. Based on the comparison of acid resistance and bile salt resistance, in vitro antioxidant capacity, and sensitivity to antibiotics, a strain with inhibitory α-glucosidase activity was screened out through in vitro tests. The strain with the strongest ability was identified as Lactobacillus plantarum Grx16 (Lactobacillus plantarum Grx16) by physiological and biochemical tests, API reagent strips and 16s rDNA sequencing analysis. The strain can be used to prepare fermented milk with the ability to inhibit the activity of α-glucosidase.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the isolation, screening method and identification of lactic acid bacteria having an inhibitory effect on alpha-glucosidase. Background technique [0002] Diabetes mellitus is a group of systemic metabolic diseases characterized by hyperglycemia, which is a symptom caused by defective insulin secretion or impaired biological action, or both. With the development of the world economy and the extension of human life expectancy, the number of diabetic patients is also increasing day by day. Diabetes has become a global threat. Drugs and insulin replacement have been widely used in the treatment of diabetes, but the side effects of drugs may lead to other symptoms such as physical discomfort, even direct use of insulin injections can also cause muscle atrophy at the injection site, blurred vision and other side effects, which makes drug treatment in Clinical applications are largely lim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/25
Inventor 顾瑞霞石雪晨印伯星孟茜陈霞黄玉军瞿恒贤蒋欣荣瓦云超陈大卫
Owner 上海昊岳生物科技有限公司
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