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A kind of Alternaria and its application in Dendrobium officinale against leaf spot

A technology of Alternaria and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of low average yield, slow growth rate of Dendrobium officinale, occurrence of diseases, etc., and achieves the effects of easy preservation, good development and application prospects, and simple culture conditions.

Active Publication Date: 2018-03-30
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield, and serious disease occurrence

Method used

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  • A kind of Alternaria and its application in Dendrobium officinale against leaf spot
  • A kind of Alternaria and its application in Dendrobium officinale against leaf spot
  • A kind of Alternaria and its application in Dendrobium officinale against leaf spot

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Embodiment 1

[0020] Analysis of the effect of Alternaria alternata JSNL005-2 on the disease resistance of Dendrobium officinale.

[0021] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL005-2 under aseptic conditions, and then carry out artificial cultivation. The specific culture conditions are 28° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 30 to 35 days. Process the cultured bacterial agent into 1cm 3 Blocks of large or small sizes are placed on the surface of the cultivation substrate of Dendrobium candidum seedlings. The inoculation amount of solid bacterial agent for each clump of Dendrobium candidum seedlings is 5g. A...

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Abstract

The invention discloses an Alternaria alternata, which is classified as Alternaria alternata and has a strain number of JSNL005-2. Committee General Microbiology Center, the deposit number is CGMCC No.11312, and the deposit date is September 11, 2015. The Alternaria JSNL005-2 of the present invention has remarkable effects in improving the growth and resistance to leaf spot of Dendrobium officinale, and can be artificially cultivated, has a fast reproduction speed, is convenient for large-scale production, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an Alternaria bacterium and its application in dendrobium candidum for resisting leaf spot. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Studies have shown that from seed germination to healthy growth of seedlings, there is no need for the participation of symbiotic fungi. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield and serious disease occurrence. At present, disease control still mainly relies on pesticides. From the perspective of protecting the ecological environment and food safety, Dendrobium candidum, as a precious traditional Chinese medicine, should reduce the applica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 徐超张红岩贾君汪善峰席刚俊赵桂华杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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