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A kind of Agaricus dwarf and its application in Dendrobium officinale against leaf spot

A technology of Dendrobium officinale and microbial strains, applied in the field of microorganisms, can solve the problems of low average yield, slow growth rate of Dendrobium officinale, occurrence of diseases, etc., and achieves the effects of easy preservation, good development and application prospects, and simple culture conditions.

Active Publication Date: 2016-09-21
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield, and serious disease occurrence

Method used

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  • A kind of Agaricus dwarf and its application in Dendrobium officinale against leaf spot
  • A kind of Agaricus dwarf and its application in Dendrobium officinale against leaf spot
  • A kind of Agaricus dwarf and its application in Dendrobium officinale against leaf spot

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Embodiment 1

[0020] Analysis of the effect of Umbelopsis nana JSNL001-3 on the disease resistance of Dendrobium officinale.

[0021] (1) Using cottonseed husk slag as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it into an autoclave bag, under high pressure conditions of 121 ° C and 101.3 kPa. Sterilize for 20 minutes. After the culture medium was cooled, the mycorrhizal fungus JSNL001-3 was inoculated under sterile conditions, and then artificially cultured. The specific culture conditions were 28°C, 12h light + 12h darkness, pH 6.5, and cultured for 25 to 35 days. Process the cultured inoculum into 1cm 3 The size of the block is placed on the surface of the Dendrobium officinale seedling cultivation substrate. The solid inoculum inoculum of each cluster of Dendrobium officinale seedlings is 5g. After the inoculum is applied, the relative water content of the substrate is m...

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Abstract

The invention discloses a mushroom called Umbelopsis nana, which is classified as Zygomycota Mucorales and Umbelopsis nana. Committee General Microbiology Center, the deposit number is CGMCC No.9565, and the deposit date is August 15, 2014. The Agaricomycetes dwarf JSNL001‑3 of the present invention has remarkable effects in improving the growth and resistance to leaf spot of Dendrobium candidum, can be artificially cultivated, has a fast reproduction speed, is convenient for large-scale production, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to agaricus dwarf and its application in anti-leaf spot disease of Dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the orchid family Dendrobium genus. Studies have shown that from the germination of its seeds to the healthy growth of seedlings, the participation of symbiotic fungi is not required. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation. At present, the control of disease still mainly relies on pesticides. From the perspective of protecting the ecological environment and food safety, Dendrobium officinale, as a precious traditional Chinese medicine, should reduce the application amount of pesticides and chemical fertilizers as much as possible. Mycorrhiza is a symbiosis with spe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 徐超席刚俊张红岩赵桂华何任红史俊郑凯杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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