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Seedling method for paris polyphylla

A technology of Aescinus aesculus and rhizomes, which is applied in the field of rapid induction of seedlings from Aescinus aescini, can solve the problems of difficult propagation of seeds and seedlings, high dormancy, and low emergence rate, and achieve rapid adaptation to the natural environment, Guaranteed genetic stability and high transplant survival rate

Active Publication Date: 2015-12-09
江西厚达科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some key technologies that need to be overcome in the artificial cultivation of Aescin
[0003] Aescinus aesculus has biological characteristics such as high degree of dormancy, slow germination, and low emergence rate, so it is difficult to propagate seeds and seedlings; rhizome propagation is not only due to the use of vegetative bodies for reproduction, but also the large demand for Aescinus aesculus rhizomes, which makes wild resources sharp. and it will degrade the germplasm and reduce the stress resistance; so far, no satisfactory results have been obtained in the tissue culture of Aescin
[0015] So far, there have been no reports at home and abroad about drug-induced formation of multiple independent buds directly targeting the rhizomes of Aescinus aesculus on ordinary seedling beds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Choose the fresh rhizomes of Aesculus aesculata excavated on January 20, and wash them with 7% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots, and the rhizome without bud head is used for medicinal purposes. Cut 10 kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix them with 15 kg of sterilized 5-mesh granular activated carbon, and keep the relative humidity at 75% at 3°C ​​for 15 days at low temperature . After the low-temperature treatment finishes, spray the germ growth-promoting solution once every three days. Spray 10kg / time of bud growth-promoting solution on rhizome of Aesculus aesculus, maintain relative humidity at 80%, light intensity 400-600LX, light 10h per day, temperature at 18°C, and sprout in 28 days. After the buds grow out, treat them three times with the rooting solution. The rooting solution is an aqueous...

Embodiment 2

[0038] Choose the fresh rhizome of Aesculus chinensis excavated on February 5, and wash it with 8% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. Cut 10kg of each Aescin flower bud head vertically into 4 to 8 small pieces, and then mix them with 16kg of sterilized 4 mesh granular activated carbon, then maintain the relative humidity at 80% at 4°C, and carry out low temperature treatment for 18 days . After the low-temperature treatment finishes, spray 1.2kg of the solution for promoting germination every three days, the solution for promoting the growth of germination is the aqueous solution of 6-BA, 0.4g / LNAA, 0.06g / LGA3, 5% carbendazim containing 4g / L, Maintain relative humidity at 80%, light intensity 300-400LX, 10 hours of light per day, temperature at 19°C, and buds grow in 25 days. After the bud grows, it is processed three times with the solution...

Embodiment 3

[0040] Choose the fresh rhizomes of Aesculus aesculi that was excavated on January 20, and wash them with 9% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. Cut 10kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix them with 20kg of sterilized 3-mesh granular activated carbon, keep the relative humidity at 75% at 6°C, and carry out low-temperature treatment for 20 days . After the low-temperature treatment finishes, spray the germ growth-promoting solution once at intervals of three days. 100kg of Aesculus aesculata rhizomes were sprayed with 13kg of bud growth-promoting solution every time, and the relative humidity was maintained at 85%, the light intensity was 400-600LX, the light was 10h per day, the temperature was 16°C, and buds grew in 32 days. After the buds grow out, treat them three times with the rooting solution. The ro...

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Abstract

The invention discloses a seedling method for paris polyphylla. A large number of complete plants can be obtained by breaking dormancy of latent buds at a low temperature and then carrying out shoot-inducing and root-inducing treatment, so that large-scale industrial breeding of the paris polyphylla is achieved. According to the seedling method, the inhibiting effect of various latent buds on a single rootstock of the paris polyphylla is completely avoided; the propagation coefficient is increased to the maximal extent; the hereditary stability of the paris polyphylla variety is fully ensured; and meanwhile, the phenomenon that variation of seeds and tissue culture seedlings is easily generated is avoided. Compared with other propagation methods employing the rootstocks, the seedling method has the advantages that completely separate plants can be formed; and seedlings with a plurality of buds can also be formed.

Description

technical field [0001] The invention relates to the technical field of medicinal plant seedling cultivation, in particular to a propagation method for rapidly inducing seedlings of Aesculus aescini. Background technique [0002] Aescin is a perennial herbaceous plant, also known as unipedal lotus, one arrow, grass river car and so on. Wild Aescin is distributed in Hunan, Yunnan, Sichuan, Guizhou and other provinces. The main production areas are Xiangxi in Hunan, Central Yunnan, Western Yunnan and Northwestern Yunnan. It grows at an altitude of 1400-3100m, with an annual average temperature of 10 ~ 13 ℃, annual rainfall 850 ~ 1200mm in cool mountainous areas, mostly in evergreen broad-leaved forests, coniferous and broad-leaved mixed forests, damp places on hillsides and shrublands. Aescin germinates and grows after 5 to 6 years from the seed, and then enters the reproductive growth period. Aescin is used as medicine with rhizomes and harvested in autumn. It is slightly c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06
CPCA01G7/06A01G22/00
Inventor 王婧
Owner 江西厚达科技有限公司
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