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A preparation method of fluorescence-magnetic resonance dual-mode carbon quantum dots

A carbon quantum dot and magnetic resonance technology, applied in the fields of chemistry and biomedicine, can solve the problems of inability to detect tissues and organs, insufficient depth of tissue imaging, and inability to apply clinical disease detection, etc. The effect of shortening the relaxation time

Active Publication Date: 2017-08-18
XUZHOU MEDICAL COLLEGE
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  • Description
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Problems solved by technology

However, although this fluorescence imaging technology has high sensitivity, compared with MRI, its depth of tissue development is obviously insufficient, and it cannot detect deep tissues and organs in the body, and cannot be applied to the detection of clinical diseases.

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  • A preparation method of fluorescence-magnetic resonance dual-mode carbon quantum dots
  • A preparation method of fluorescence-magnetic resonance dual-mode carbon quantum dots
  • A preparation method of fluorescence-magnetic resonance dual-mode carbon quantum dots

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preparation example Construction

[0026] Such as figure 1 As shown, a method for preparing a fluorescent-magnetic resonance dual-mode nanoprobe of the present invention comprises the following methods:

[0027] (1) Dissolve ethylenediaminetetraacetic acid and manganese-containing compounds with a molar ratio of 1:0.05 to 1:2 in an amino compound and ethylene glycol solvent with a volume of 1:10 to 10:1. Magnetic stirring is carried out under high temperature conditions, and the reaction time is 4h-48h to obtain a dark brown homogeneous solution;

[0028] (2) Put the solution prepared in step (1) into a dialysis bag (MWCO: 1000Da) for dialysis, the dialysis time is 48h~72h, and change the water every 6h;

[0029] (3) The dialysis product in step (2) is subjected to vacuum rotary evaporation to a solid state, the evaporation temperature is 50 ° C, and the pressure is -0.1 MPa, that is, manganese-doped carbon quantum dots are obtained, which are recorded as Mn-CQDs;

[0030] The particle size of the manganese-d...

Embodiment 1

[0033] Dissolve 0.5mmol ethylenediaminetetraacetic acid and 1.0mmol manganese chloride (molar ratio 1:2) into 2mL triethylenetetramine and 5mL ethylene glycol, stir magnetically at a high temperature of 200°C, and the reaction time is 4h. Obtain a dark brown homogeneous solution; put the gained solution into a dialysis bag (MWCO: 1000Da) for dialysis, the dialysis time is 72h, and change the water every 6h; the product after the dialysis is vacuum rotary evaporated to a solid state, the evaporation temperature at 50°C and a pressure of -0.1MPa to obtain manganese-doped carbon quantum dots, denoted as Mn-CQDs;

[0034] The particle size of the manganese-doped carbon quantum dot is 1.2nm-4.9nm, and it emits bright blue fluorescence under an ultraviolet lamp (365nm), its fluorescence emission peak is at 450nm, and its relaxation efficiency is 3.07±0.26mM -1 the s -1 .

Embodiment 2

[0036] Take 0.5mmol of ethylenediaminetetraacetic acid and 1.0mmol of manganese chloride (molar ratio 1:2) into 2mL of triethylenetetramine and 5mL of ethylene glycol, and stir magnetically at 120°C for 26h to obtain dark brown uniform quality solution; put the obtained solution into a dialysis bag (MWCO: 1000Da) for dialysis, the dialysis time is 60h, and change the water every 6h; the product after dialysis is vacuum rotary evaporated to a solid state, and the evaporation temperature is 50°C. Manganese-doped carbon quantum dots were obtained at a pressure of -0.1MPa, denoted as Mn-CQDs;

[0037] The particle size of the manganese-doped carbon quantum dots is 0.7nm-4.9nm, and it emits bright blue fluorescence under an ultraviolet lamp (365nm), its fluorescence emission peak is at 436nm, and its relaxation efficiency is 2.97±0.07mM -1 the s -1 .

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Abstract

The invention discloses a method for preparing fluorescence-magnetic resonance dual-mode carbon quantum dots, which comprises the following method: dissolving a manganese-containing compound and ethylenediaminetetraacetic acid with a molar ratio of 1:0.05 to 1:2 in a volume ratio of In the amino compound and ethylene glycol solvent of 1:10-10:1, magnetic stirring is carried out at a high temperature above 100°C for 4h-48h; the obtained solution is put into a dialysis bag for dialysis, and the dialysis time is 48h-72h. The water is changed every 6h; the obtained dialysis product is subjected to vacuum rotary evaporation to a solid state, and manganese-doped carbon quantum dots are obtained, which are denoted as Mn-CQDs; the manganese-doped carbon quantum dots targeted imaging prepared by the present invention have Diversity realizes the combined use of fluorescence imaging and MRI magnetic resonance imaging technology, and the changes of fluorescence and magnetic resonance signals are displayed intuitively and easy to operate.

Description

technical field [0001] The invention specifically relates to a preparation method of fluorescence-magnetic resonance dual-mode carbon quantum dots, belonging to the technical fields of chemistry and biomedicine. Background technique [0002] Magnetic resonance imaging (MRI) is a non-invasive imaging method with high spatial resolution and tissue resolution, which can perform morphological and functional imaging on the body. Magnetic resonance nanoparticle contrast agent is a research hotspot in recent years, among which superparamagnetic iron oxide is more common. However, as a negative contrast agent, its T2 low signal is difficult to distinguish from calcification, hemorrhage, metal artifacts, etc., and its sensitivity is not as strong as that of a positive contrast agent T1 high signal, so its application in clinical work is limited. [0003] However, as a single-modality imaging technique, MRI has low sensitivity to cellular / molecular imaging, and cannot clearly display...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65G01N21/64A61K49/06
Inventor 韩翠平徐慧婷徐凯
Owner XUZHOU MEDICAL COLLEGE
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