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Method for acclimatizing and transplanting bletilla striata tissue culture seedlings

The technology of Bletilla striata group and protocorm is applied in the field of domestication and transplanting of Bletilla striata tissue culture seedlings, which can solve the problems of low survival rate, long domestication period and high cost, and achieve the effects of improving survival rate, shortening period and reducing domestication cost.

Inactive Publication Date: 2015-12-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for domesticating and transplanting bletilla striata tissue culture seedlings, so as to solve the problems of long domestication period, high cost and relatively low survival rate in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] After soaking the moss in water for 12 hours, wring it out, break it into small sections of about 1 to 2 cm with a pulverizer; put the pulverized moss, perlite and peat soil into a woven bag, and sterilize at 121°C and 103.4 kpa for 2 hours; After the sterilization is complete, take it out to dry for later use. Measure moss, perlite and peat soil according to the volume ratio of 1:2:1, mix them evenly, and spread them on a 1.4m wide seedbed in a 8m wide single greenhouse with a substrate thickness of 5-6cm, each greenhouse Place 4 seedbeds in the middle.

[0026] After strong seedlings take root, tissue culture seedlings with protocorm larger than 0.3 cm are selected for use. Pour 1 / 4 of tap water into the tissue culture bottle after planting cultivation, shake it gently, then gently take out the tissue culture plantlet together with the culture medium with tweezers, place it on a clean tray, and gently separate the plantlets from the culture medium by hand , And wash in...

Embodiment 2

[0030] After soaking the moss in water for 12 hours, wring it out, break it into small sections of about 1 to 2 cm with a pulverizer; put the pulverized moss, perlite and peat soil into a woven bag, and sterilize at 121°C and 103.4 kpa for 2 hours; After the sterilization is complete, take it out to dry for later use. Measure moss, perlite and peat soil in a volume ratio of 1:1:1, mix them evenly, and spread them on a 1.4m wide seedbed in an 8m wide single greenhouse with a substrate thickness of 5-6cm. Each greenhouse Place 4 seedbeds in the middle.

[0031] After strong seedlings take root, tissue culture seedlings with protocorm larger than 0.3 cm are selected for use.

[0032] Pour 1 / 4 of tap water into the tissue culture bottle after planting cultivation, shake it gently, then gently take out the tissue culture plantlet together with the culture medium with tweezers, place it on a clean tray, and gently separate the plantlets from the culture medium by hand , And wash in cle...

Embodiment 3

[0036] After soaking the moss in water for 12 hours, wring it out, break it into small sections of about 1 to 2 cm with a pulverizer; put the crushed moss and peat soil into a woven bag, and sterilize at 121°C and 103.4kpa for 2 hours; the sterilization is complete After that, take it out to dry for later use. Measure moss and peat soil in a volume ratio of 1:1, mix them evenly, and spread them on a 1.4m wide seedbed in an 8m wide single greenhouse with a substrate thickness of 5-6cm, and place 4 seedbeds in each greenhouse .

[0037] After strong seedlings take root, tissue culture seedlings with protocorm larger than 0.3 cm are selected for use.

[0038] Pour 1 / 4 of tap water into the tissue culture bottle after planting cultivation, shake it gently, then gently take out the tissue culture plantlet together with the culture medium with tweezers, place it on a clean tray, and gently separate the plantlets from the culture medium by hand , And wash in clean water to thoroughly wa...

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Abstract

The invention discloses a method for acclimatizing and transplanting bletilla striata tissue culture seedlings. The method includes the step that the bletilla striata tissue culture seedlings are sequentially soaked in a mancozeb aqueous solution and rooting water to be acclimatized and transplanted on acclimatizing matrixes. Compared with the prior art, the method has the advantages that seedling hardening is avoided, the bletilla striata tissue culture seedlings can rapidly adapt to the natural environment, and the acclimatizing and transplanting cycle can be shortened. In addition, peat soil, perlite and moss are selected as the acclimatizing matrixes; compared with the prior art, the better water retaining capacity and the better air permeability are achieved, the acclimatizing cost is reduced, and meanwhile damage to the ecological environment is reduced. Meanwhile, the bletilla striata tissue culture seedlings are soaked through the rooting water, original bulbs can grow new roots and new buds in a short cycle, and the transplanting survival rate of the tissue culture seedlings is increased and is higher than 95%.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine cultivation and domestication, and specifically relates to a domestication and transplantation method of Bletilla striata tissue culture seedlings. Background technique [0002] Bletillastriata (Bletillastriata), also known as white and white root, ground screw, is a perennial herbaceous white and orchid family. Bletilla striata grows in evergreen broad-leaved forests with an altitude of 100-3200m. It likes a warm, humid environment and is slightly hardy. Bletilla striata is a traditional and precious Chinese medicine in our country. It uses dried tubers as medicine. Its nature and flavor are: bitter, sweet, astringent, and slightly cold. It has the effects of astringent, hemostasis, swelling and muscle growth, and can be used to treat internal and external bleeding, burns, chapped hands and feet, and carbuncle. Bletilla striata contains Bletilla striata polysaccharide, bibenzyl, phenanthr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00
CPCA01G24/00A01G2/00A01G24/35A01G31/00
Inventor 席刚俊徐超史俊汪善锋贾君杨鹤同李警保赵桂华郑凯
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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