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A kind of transplanting method of peanut tissue culture seedling

A technology for tissue culture seedlings and peanuts is applied in the field of transplanting peanut tissue culture seedlings, which can solve the problems of long culture time, easy contamination, and large space occupation, and achieve the effect of improving the survival rate.

Active Publication Date: 2017-10-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for transplanting peanut tissue-cultured seedlings, which can solve the problem that grafted regenerated seedlings need to be domesticated in a domestication room, occupy a large space, and take a long time to cultivate.

Method used

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  • A kind of transplanting method of peanut tissue culture seedling
  • A kind of transplanting method of peanut tissue culture seedling
  • A kind of transplanting method of peanut tissue culture seedling

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Experimental program
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Embodiment 1

[0033] The transplanting method of a kind of peanut tissue culture seedling of the present invention specifically comprises the steps:

[0034] 1. Using peanut varieties Huayu 20 (provided by Shandong Peanut Research Institute), Huayu 22 (provided by Shandong Peanut Research Institute), and Luhua 11 (provided by Qingdao Agricultural University) embryo leaflets as explants, regeneration obtained through embryogenesis The seedlings are used as scions, and the peanut seedlings germinated in the sand are used as rootstocks for grafting.

[0035] The peanut seedlings used as rootstocks are as follows: peanut seeds are surface-sterilized, placed in a high-temperature and high-pressure sterilized culture bottle filled with sand, the seeds are buried in the sand, and the seeds are covered with sand with a thickness of 2 to 3 cm. About 11 to 13 days after germination, the seedlings grown from the seeds are the peanut seedlings (such as figure 1 ), the grafting interface is below the c...

Embodiment 2

[0046] The transplanting method of peanut tissue culture seedling of the present invention specifically comprises the steps:

[0047] 1. Use the cotyledons of Huayu No. 23 (provided by Shandong Peanut Research Institute) as transgenic explants, and regenerate seedlings obtained by transforming chitinase gene and β-1,3-glucanase gene respectively as scions. Germinated peanut seedlings were grafted as stock; the peanut seedlings were obtained as shown in Example 1.

[0048] 2. Transplant the grafted regenerated seedlings directly into the field. The spacing between the rows is 40-45cm, and the spacing between the plants is 16-20cm. Sandy loam is suitable;

[0049] 3. Build a plastic bow shed on the grafted regenerated seedlings;

[0050] 4. Water enough water to supply the water needs of transplanted seedlings, and ensure that the air humidity in the plastic shed is above 80%.

[0051] 5. Set up a shade net from 9:00 am to 4:00 pm in the first 2 weeks after transplanting the ...

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Abstract

The invention provides a transplanting method for tissue-cultured peanut seedlings. The transplanting method comprises the main steps: taking peanut regeneration seedlings as scions and taking peanut seedlings subjected to germination acceleration in sterile sand as stocks to be grafted to obtain grafted regeneration seedlings; directly transplanting the grafted regeneration seedlings into a large field and irrigating enough water; erecting a plastic arch-shaped shed above the grafted regeneration seedlings to keep the air humidity; accepting light illumination in the morning and afternoon at first two weeks when the seedlings are transplanted and erecting a shading net at other daytime; after 2 weeks, removing the shading net and letting in fresh air to avoid excessive temperature in the plastic arch-shaped shed; meanwhile, carrying out seedling hardening; after transplanting for 3 weeks, removing the plastic arch-shaped shed and carrying out field management of the grafted regeneration seedlings according to common field management. According to the technical scheme provided by the invention, the problems that the grafted regeneration seedlings need to be domesticated in a domestication room, the occupied space is large, the culture time is long, and the grafted regeneration seedlings grow slowly and are easy to contaminate so that the survival rate is low can be solved.

Description

technical field [0001] The invention relates to a transplanting method of peanut tissue culture seedlings, in particular to a transplanting method of peanut tissue culture seedlings. Background technique [0002] Peanut is one of the important oil crops and economic crops in my country, but its genetic base is narrow, its genetic diversity is poor, and it lacks high-quality germplasm resources such as high resistance, high oil and high protein. Genetic transformation, in vitro mutagenesis, and cell fusion can create new peanut germplasm resources, broaden the genetic basis of peanut, and provide materials for peanut breeding. However, the peanut tissue culture seedlings are difficult to take root, the survival rate of transplanting is low, and the transplanting seedlings are not easy to bear fruit. The application of grafting technology can solve these problems. But generally the regenerated seedlings of grafting are first cultivated in the domestication room, and can be t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G1/06
CPCA01G2/30A01G22/00
Inventor 王晶珊李冠张丹丹隋炯明乔利仙姜德锋盖树鹏
Owner QINGDAO AGRI UNIV
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