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PK cell culture medium

A culture medium and cell technology, applied in the field of biomedicine, can solve the problems of high cost and complicated materials, and achieve the effects of strong applicability, improved efficiency, and reduced production costs

Inactive Publication Date: 2015-11-25
上海源培生物科技股份有限公司
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  • Claims
  • Application Information

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Problems solved by technology

Nowadays, the materials used for PK cell culture medium in the market are more complicated and the cost is higher

Method used

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Embodiment Construction

[0034] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention will be further explained below.

[0035] A PK cell culture medium, based on the final concentration, the formula includes: 4-hydroxyethylpiperazine ethanesulfonic acid 3000mg / L~4000mg / L, sodium chloride 6000mg / L~7000mg / L, D-(+)- Glucose 3000mg / L~4000mg / L, sodium bicarbonate 1000mg / L~2000mg / L, reduced glutathione 0.01mg / L~10mg / L, ferric ammonium citrate 0.001mg / L~10mg / L and uracil 0.01mg / L-10mg / L. In the present invention, 4-hydroxyethylpiperazine ethanesulfonic acid and sodium bicarbonate are added to the formula to act as buffers to maintain the constant pH value in the culture medium. 4-Hydroxyethylpiperazine ethanesulfonic acid acts as a buffer, and D-(+)-glucose acts as a reducing agent in the medium. The role of sodium chloride is to maintain the osmotic pressure in the medium. The invention optimizes the formula of a t...

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Abstract

The invention relates to the field of biomedicine. According to final concentration, the PK cell culture medium consists of the following components in a formula: 3000mg / L-4000mg / L of 4-hydroxyethyl piperazine ethanesulfonic acid, 6000mg / L-7000mg / L of sodium chloride, 3000mg / L-4000mg / L of D-(+)-glucose, 1000mg / L-2000mg / L of sodium bicarbonate, 0.01mg / L-10mg / L of reduced glutathione, 0.001mg / L-10mg / L of ammonium ferric citrate and 0.01mg / L-10mg / L of uracil; and the sodium chloride takes an effect of maintaining osmotic pressure inside the culture medium. By optimizing a conventional formula of the PK cell culture medium, the culture medium disclosed by the invention can save production cost and can improve the efficiency of cell culture, and the culture medium is more suitable for the growth of animal cells. The culture medium disclosed by the invention is applicable to VERO, PK15 and MAC145 cells, and is strong in applicability.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a serum-free culture medium. Background technique [0002] PK (pig kidney) cell culture medium is a synthetic medium that can maintain the growth and reproduction of cells in vitro for a long time without adding serum. It is composed of a variety of amino acids, vitamins, trace elements and inorganic compounds, and provides optimization for animal cells. The growth environment. The production of PK cell culture media is a basic product in the biomedical industry. With the upgrading of technology in the biopharmaceutical and biomedical industries, cell culture technology is constantly evolving, and the amount of serum is gradually reduced until the use of serum to culture animal cells is stopped. Manufacturers need to continuously innovate technologies and develop high-quality PK cell culture media products to meet the needs of downstream customers. Nowadays, the PK cell culture media on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 温韬方芳肖杰
Owner 上海源培生物科技股份有限公司
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