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Preparation method and application of chitobiose

A technology of oligochitosan and chitosan, which is applied to the preparation of sugar derivatives, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problems of poor activity of oligochitosan, achieve good results, improve The effect of utilization

Active Publication Date: 2015-11-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the existing chitosan oligosaccharides have poor activity in inhibiting tumors, and according to the existing domestic and foreign references, the research on the administration method and dosage of chitosan oligosaccharides in vivo tumor antagonism is still blank. Few reports have been reported on the screening of inhibitory effects of sugars on multiple tumor cell lines

Method used

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  • Preparation method and application of chitobiose
  • Preparation method and application of chitobiose
  • Preparation method and application of chitobiose

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Experimental program
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Effect test

specific Embodiment approach 1

[0023] Specific embodiment 1: a preparation method of chitosan oligosaccharide in this embodiment is specifically prepared according to the following steps:

[0024] Dissolving chitosan in a glacial acetic acid solution with a mass concentration of 0.1-5.0%, then adding 5-25 times the weight of chitosan with a concentration of 10%-40% hydrogen peroxide, heating and stirring to a constant temperature of 40-90°C, The reaction is carried out for 0.5 to 10 hours, and then precipitated by ethanol with a mass concentration of 60 to 100%, filtered, and the filter residue is dried to obtain chitosan oligosaccharide with a degree of polymerization of 2 to 30; wherein the content of the chitosan oligosaccharide with a degree of polymerization of 2 to 10 is not less than 80%, the purity of chitosan oligosaccharide excluding moisture content is >98%; the mass-volume ratio of chitosan to glacial acetic acid solution with a mass concentration of 0.1-5.0% is 1 g:(10-30) mL.

[0025]This embo...

specific Embodiment approach 2

[0026] Embodiment 2: The difference between this embodiment and Embodiment 1 is that chitosan is dissolved in a glacial acetic acid solution with a mass concentration of 3.5%, and then 20 times the concentration of chitosan is added to a concentration of 30% hydrogen peroxide . Others are the same as the first embodiment.

specific Embodiment approach 3

[0027] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the temperature is raised and stirred to a constant temperature of 70°C, and the reaction is performed for 8 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a preparation method and application of chitobiose. The preparation method comprises the following steps of dissolving chitobiose in glacial acetic acid solution; adding hydrogen peroxide; heating and stirring until the temperature is 40-90 DEG C and incubating; precipitating with ethanol after reaction is finished; filtering; and drying filter residues to obtain the chitobiose. The prepared chitobiose is used for preparing medicines for suppressing 10 strains of tumor cells of 5 kinds of humanized tumors. Biological compatibility and tumor atagonistics activity of the chitobiose are utilized effectively. By analyzing antitumor activity of the chitobiose, development of anti-tumor medicaments is promoted. The invention is used for the field of anti-tumor medicines.

Description

technical field [0001] The present invention relates to a preparation method of chitosan oligosaccharide and its application. Background technique [0002] Chitin is widely present in the shells of crustaceans, the shells of insects and the cell walls of fungi. It is the most abundant natural polymer in nature, and its content is second only to cellulose. Chitin has a variety of functional properties, but its poor water solubility and biodegradability greatly limit its wide application. Compared with the characteristics of chitin being insoluble in water, dilute acid, dilute alkali, ethanol or other organic solvents, chitosan is soluble in water and exhibits higher viscosity in dilute acid solution. This solubility property also limits the application of chitosan in the biological field. Also as a chitin degradation product, chitosanoligosaccharides (COS) have better solubility and lower viscosity under physiological conditions, due to the shorter sugar chain and free amin...

Claims

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Application Information

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IPC IPC(8): C08B37/08C07H1/00C07H5/06A61K31/722A61K31/7016A61K31/702A61P35/00
Inventor 杨鑫邹攀王静
Owner HARBIN INST OF TECH
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