Method for preparing pure black fungus or pure kelp broth product based on thermal explosion pretreatment
A pretreatment and fermentation liquid technology, which is applied in the field of preparation of pure black fungus or pure kelp fermentation liquid products based on thermal explosion pretreatment, can solve the problems of long time consumption, unstable quality, disadvantages, etc., and achieve low investment, simple equipment and low loss small effect
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Embodiment 1
[0021] Choose 20g of dried black fungus without pests, wash the sediment, add 45°C water to soak, and leave the open water after fully absorbing water. It is pulverized according to the size of 2-3mm in diameter. The raw material is passed through a screw extruder, and the screw is heated with four-stage temperature gradients of 80, 110, 140, and 170°C; extruded to obtain puffed raw materials with an expansion ratio of 3.8 or more. Add 500 milliliters of water to the black fungus after the above treatment, stir and heat (maintain vapor pressure 0.2 MPa) for 35 minutes at 125-135° C., and measure the degree of saccharification to reach 20° BX. The black fungus liquid treated above was cooled to 25° C., and 2 g of strain Lactobacillus bulgaricus was added thereto. Stirring fermentation at 50°C: 1 day. Obtain black fungus fermented liquid. The puree was centrifuged at 4000 rpm for 15 minutes, and the wet residue was 2 g; the supernatant was filtered through a 0.8 μm ceramic me...
Embodiment 2
[0035] Choose 120g of freshly picked black fungus, wash the sediment and leave the open water. It is pulverized according to the size of 2-3mm in diameter. The raw materials are passed through a screw extruder, and the four-stage temperature gradient of the screw heating is 90, 110, 130, 150°C; extruded to obtain puffed raw materials with an expansion ratio of 2.8 or more. Add 350 milliliters of water to the black fungus after the above treatment, and stir and heat for 35 minutes at 100° C., and the degree of saccharification reaches 20° BX as measured. The black fungus liquid treated above was cooled to 25° C., and 4 g of Lactobacillus crispatus was added thereto. Stirring and fermentation at 50°C: 2 days. Obtain black fungus fermented liquid. The puree was centrifuged at 4000 rpm for 15 minutes, and the supernatant of 3 g of the wet residue was filtered through a 0.8 μm ceramic membrane to obtain 260 ml of small-molecule puree of fungus fermentation broth and 70 ml of con...
Embodiment 3
[0045] Choose 40g of dry kelp without pests, wash the sediment, add 35 ℃ water to soak, and leave the open water after fully absorbing water. It is pulverized according to the size of 2-3mm in diameter. The raw material is passed through a screw extruder, and the screw is heated with four-stage temperature gradients of 90, 110, 130, and 160°C; extruded to obtain puffed raw materials with an expansion ratio of 4.8 or more. Add 500 milliliters of water to the above-mentioned treated kelp, stir and heat (maintain vapor pressure 0.2 MPa) for 35 minutes at 125-135° C., and measure the degree of saccharification to reach 20°BX. Cool the above-mentioned kelp liquid to 25°C, add 4g of Lactobacillus fermentum and 4g of Lactobacillus gratuitatus to it (keep at 50°C, stir and ferment for 10 days. Obtain kelp koji fermentation liquid. Distill the puree at 4000 Spin / min and centrifuge for 15 minutes, filter the supernatant of 8 g of wet residue with a 0.8 μm ceramic membrane, and obtain 4...
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