Method for breeding double recessive homozygous sweet-waxy maize hybrid through sh2 gene type super-sweet maize mutant with high starch content

Abstract

The invention discloses a method for breeding double recessive homozygous sweet-waxy maize hybrid through a sh2 gene type super-sweet maize mutant with high starch content. The collection number of the sh2 gene type super-sweet maize mutant V240TB with the high starch content is CGMCC No.10410. The sh2 gene type super-sweet maize mutant V240TB with the high starch content is hybridized with ordinary waxy maize, and a double recessive homozygous sweet-waxy maize inbred line with the high starch content is obtained. Two double recessive homozygous sweet-waxy maize inbred lines with the high starch content are matched, and the double recessive homozygous sweet-waxy maize hybrid is obtained and has higher sugar content than the ordinary waxy maize and higher starch content than ordinary super-sweet maize, and the starch is all amylopectin. The bred double recessive homozygous sweet-waxy maize hybrid inbred line and hybrid with the high starch content integrate respective advantages of sweet maize and waxy maize, the sweetness is high, the waxiness is good, the maize kernels have identical appearance and maturity, and meanwhile, the seed germination percentage reaches the standard of ordinary maize.

Description

technical field [0001] The invention belongs to the technical field of plant genetics and breeding. The sh2 genotype super-sweet corn with more starch-containing mutant is used to introduce the wx gene of waxy corn through hybridization, and the double-recessive homozygous sh2 gene and wx gene with more starch are selected. Finally, a double-recessive homozygous sweet-waxy corn hybrid was bred, which not only contained more amylopectin than ordinary super-sweet corn, but also contained more sugar than ordinary waxy corn. Background technique [0002] Super-sweet corn shrunken2 gene: Compared with ordinary sweet corn (su genotype), super-sweet corn has a more complex genetic background and multiple genotypes (sh1, sh2, bt1, bt2), among which the most typical and practical The most utilized in maize breeding is the recessive mutant gene shrunken2. In 1953, Laughnan discovered the mutant gene, which was located on the long arm of the third chromosome of maize. Research shows t...

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Problems solved by technology

[0008] 2. The seeds of double-recessive or triple-recessive lines appear as sweet corn kernels, shriveled and shriveled. Although the total sugar content is higher than that of super sweet corn, the starch content of endosperm after the seeds mature is less than that of super sweet corn, so they germinate Low yield, difficult seed germination, increased technical difficulty in the process of parental reproduction and hybrid seed production

[0009] 3. Because the main components of waxy corn kernels and sweet corn kernels are different, the filling speed and maturity period of the two kinds of kernels are different, which makes it difficult to choose the best harvest time, and the composition of the two kinds of kernels is quite different. Super-sweet grains have high sugar content, high water content, and low starch content. On the contrary, waxy grains have low sugar content, low water content, and high starch content (amylopectin). The difference between the two types of grains can be seen directly after the ears are processed. poor visual effect

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