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Breeding method for a cytoplasmic male sterile restorer line of bud yellow pepper

A technology of male sterile lines and capsicum cytoplasm, applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the difficulty of screening large fruit type restorer line materials, consume manpower, material and financial resources, and have a large workload To achieve the effect of controllable breeding target traits and procedures, simple breeding procedures, and improved breeding efficiency

Active Publication Date: 2017-11-28
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the distribution of restorer line materials in different types of peppers is different, and the identification is mainly through a large number of paired side crosses, the workload is large and the efficiency is not high. In small-fruited peppers such as croissant and threaded peppers, it is even more difficult to screen large-fruited restorer materials, especially using a large number of paired side crosses for identification and screening, which not only consumes a lot of manpower, material and financial resources, but also reduces the efficiency. very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Breeding of Restorer Line of Bud Yellow Pepper Cytoplasmic Male Sterile Line 140-6A

[0022] Step 1. Pollen collection: In the spring of 2011, the bud-yellow pepper cytoplasmic male sterile line 140-6A was used as the female parent, and the fruit was cone-shaped and sweet. With 100 inbred line peppers as the male parent as 1 group, a total of 3 groups, 300 male parent materials, divided into groups A, B and C, of ​​which groups A and B are peppers, and group C is sweet pepper; For the collection of pollen, take the pollen directly from the male parent flower in full bloom on that day, collect 2 flower pollen from each male parent, and collect 100 male parent pollen at a time as one group, divided into 3 groups, and collect the male parent pollen Use a shaker to mix evenly, and directly pollinate after numbering;

[0023] Step 2. Pollination: Pollinate the sterile line 140-6A with 3 sets of numbered mixed pollen. The pollination adopts the flowers that bloom on the same day ...

Embodiment 2

[0029] Breeding of Restorer Line of Bud Yellow Pepper Cytoplasmic Male Sterile Line 140-2A

[0030] Step 1. Pollen collection: In the spring of 2011, the bud-yellow pepper cytoplasmic male sterile line 140-2A was used as the female parent (its genotype was S(rfrf)), the fruit type was horn type, and the spicy taste was slightly spicy. Take 50 inbred line peppers as male parent (genotype unknown material) as 1 group, a total of 3 groups, 150 male parent materials, divided into groups A, B and C, of ​​which groups A and B are peppers, and C The group is sweet pepper; the male parent pollen is collected directly from the male parent flower in full bloom that day, each male parent collects 2 flower pollen, a single collection of 50 male parent pollen is a group, divided into 3 groups, Mix the collected male parent pollen with a shaker, and after numbering, directly pollinate;

[0031] Step 2. Pollination: Pollinate the male sterile line 140-2A with three groups of numbered mixed polle...

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Abstract

The invention discloses a method for breeding the male sterility restoring line of yellow sprout pepper cytoplasm and belongs to the technical field of plant breeding. The method includes the specific steps that mixed pollination is conducted on the male sterility restoring line of the yellow sprout pepper cytoplasm and selfing line pepper male parent pollen, harvested hybrid seeds are sowed, the fertile plant line and fertile plants are screened many times, strict selfing is conducted, and the male sterility restoring line of the yellow sprout pepper cytoplasm with the restoring rate being 100% is obtained finally; compared with an existing breeding technology of the pepper male sterility restoring line, the method has the advantages that the method is simple in breeding procedure, convenient to implement and controllable in target trait and procedure of breeding; the breeding efficiency is improved greatly, the breeding restoring line type and number are increased greatly, and a new purpose is developed for advantage utilization of pepper crossbreed, leaf color marking sterility restoring line breeding and the like.

Description

Technical field [0001] The invention relates to a breeding method of pepper, in particular to a breeding method of a cytoplasmic male sterile restorer line of yellow pepper, belonging to the technical field of plant breeding. Background technique [0002] Studies have shown that there is an obvious heterosis among the hybrids of pepper varieties. The traditional ways to utilize the heterosis of peppers include artificial emasculation interspecific hybridization, double crosses, triple crosses, male sterile line seed production, and chemical emasculation seed production. Breeding new varieties using dual-purpose male sterile lines and cytoplasmic male sterile lines has become the focus of pepper breeding. Because the pepper cytoplasmic male sterile line can produce 100% sterile plants, it can greatly improve the purity of the first hybrid seed, and it has a high production significance. However, due to the different distribution of restorer line materials in different types of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 马志虎孙国胜孙春青潘跃平
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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