Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line
A technology of recessive epistatic interaction and Brassica napus, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding cycle and difficulty in breeding, and achieve simple breeding procedures, The effect of strong operability and improving breeding efficiency
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Embodiment 1
[0018] (1) Recessive genic male sterile two-line male sterile line B strain 09105B ( BnMs 1 ms 1 ms 2 ms 2 or Bnms 1 ms 1 Mrs. 2 ms 2 ) Select 3 bagged plants for self-crossing, plant them into 3 plots, and plant about 200 plants in each plot, among which the fertile plants in the first plot: sterile plants = 152:60, and the fertile plants in the second plot: sterile Plants = 167:65, fertile plants in the third plot: sterile plants = 158:44, in which the performance of the second plot was relatively good, and 10 fertile plants were selected for self-bagging.
[0019] (2) Plant the 10 individual plant seeds obtained in step (1) into 10 different plots, plant about 100 plants in each plot, of which 4 plots have no fertility separation, and the fertility separation ratio of 6 plots is close to 3: 1. Select a plot with excellent performance from the 4 plots separated by sterility, and harvest 5 self-bred single-plant seeds from this plot 09105B ( BnMs 1 M...
Embodiment 2
[0030] (1)~(6) The basic steps are the same as in Example 1, but the material is 08112B, which is the B strain of Huangzi double-low recessive genic sterile two-line male sterile line.
[0031] (7) Step (6) The self-bred offspring were planted into 50 different plots, and about 200 plants were planted in each plot. It was found that the fertility segregation ratio of 7 plots was close to 13:3, and one excellent plot was selected, from which Select 50 fertile plants for self-bagging.
[0032] (8) Step (7) The self-bred progenies were planted into 50 different plots, and it was found that the fertility segregation ratio of 8 plots was close to 3:1. One of the excellent plots was selected, and 10 pairs of test crosses were selected.
[0033] (9) The test cross progeny of step (8) was planted into 10 plots, and about 200 plants were planted in each plot. It was found that the fertility separation ratio of the test cross progeny in 7 plots was close to 1:1, and 3 plots were sterile...
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