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Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line

A technology of recessive epistatic interaction and Brassica napus, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding cycle and difficulty in breeding, and achieve simple breeding procedures, The effect of strong operability and improving breeding efficiency

Inactive Publication Date: 2014-07-23
贵州省油菜研究所
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  • Application Information

AI Technical Summary

Problems solved by technology

The vast number of rapeseed breeding scientific and technological workers use this as the basic material to genetically improve the three-line recessive genetic male sterile material, but the breeding cycle of each rapeseed breeding unit is long, which is rare. Since researcher Chen Fengxiang discovered the recessive nuclear male sterile After 30 years of breeding practice, only a dozen of three-line male sterile varieties have been approved and promoted nationwide.

Method used

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  • Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line
  • Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line
  • Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line

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Effect test

Embodiment 1

[0018] (1) Recessive genic male sterile two-line male sterile line B strain 09105B ( BnMs 1 ms 1 ms 2 ms 2 or Bnms 1 ms 1 Mrs. 2 ms 2 ) Select 3 bagged plants for self-crossing, plant them into 3 plots, and plant about 200 plants in each plot, among which the fertile plants in the first plot: sterile plants = 152:60, and the fertile plants in the second plot: sterile Plants = 167:65, fertile plants in the third plot: sterile plants = 158:44, in which the performance of the second plot was relatively good, and 10 fertile plants were selected for self-bagging.

[0019] (2) Plant the 10 individual plant seeds obtained in step (1) into 10 different plots, plant about 100 plants in each plot, of which 4 plots have no fertility separation, and the fertility separation ratio of 6 plots is close to 3: 1. Select a plot with excellent performance from the 4 plots separated by sterility, and harvest 5 self-bred single-plant seeds from this plot 09105B ( BnMs 1 M...

Embodiment 2

[0030] (1)~(6) The basic steps are the same as in Example 1, but the material is 08112B, which is the B strain of Huangzi double-low recessive genic sterile two-line male sterile line.

[0031] (7) Step (6) The self-bred offspring were planted into 50 different plots, and about 200 plants were planted in each plot. It was found that the fertility segregation ratio of 7 plots was close to 13:3, and one excellent plot was selected, from which Select 50 fertile plants for self-bagging.

[0032] (8) Step (7) The self-bred progenies were planted into 50 different plots, and it was found that the fertility segregation ratio of 8 plots was close to 3:1. One of the excellent plots was selected, and 10 pairs of test crosses were selected.

[0033] (9) The test cross progeny of step (8) was planted into 10 plots, and about 200 plants were planted in each plot. It was found that the fertility separation ratio of the test cross progeny in 7 plots was close to 1:1, and 3 plots were sterile...

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Abstract

The invention discloses a method for breeding two-type lines of recessive epistatic interaction genic male sterile in Brassica napus. The method selects excellent recessive genic male sterile two-line male sterile line B strains for self-bagging, and obtains self-bred sterile Fertility segregated rapeseed plants; the obtained self-sterility segregated rapeseed plants are recurrent parents, which are backcrossed with the recessive epistasis inter-GMS two-type line, and self-crossed and testcrossed in the backcrossed progeny population to obtain A new recessive epistatic interaction genic homozygous two-type line; the recessive epistatic interaction genic homozygous two-type line was test crossed with the Lingbao line, and a total sterile line with 100% sterile plants was obtained. The invention has the advantages of simple breeding procedure, fast speed and strong operability, and the obtained homozygous two-type line has stable genetics and high combining ability, and can be conveniently applied to the breeding of new varieties of recessive epistatic interaction sterility hybrid rapeseed.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a new method for breeding two-type lines of recessive epistatic interaction genic male sterile in Brassica napus. Background technique [0002] Rapeseed has strong heterosis, and good hybrid combinations can generally increase yield by 20% to 30%. Since the first approved and widely planted hybrid rapeseed Qinyou No. 2 was bred in 1985, the production of hybrid rapeseed has developed rapidly in my country. At present, the way of rapeseed hybrid utilization is mainly the male sterility of rapeseed. The key to the utilization of rapeseed male sterility is to obtain 100% sterile population during hybrid production, and the recessive nuclear sterile two lines can only obtain 50% during hybrid production. For sterile groups, the production of hybrids is time-consuming and labor-intensive, and the purity of the seeds is difficult to guarantee, and the yield of se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 伍林涛杜才富韩宏仕奉斌张敏琴曾章丽
Owner 贵州省油菜研究所
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