A method for creating a cytoplasmic male sterile line of pepper and matching three lines at the same time

Abstract

The invention discloses a method for developing pepper cytoplasm male sterile lines and simultaneously matching three lines. The method comprises the following steps: taking a material with genotype S(RfRf) as a female parent, taking a material with genotype N(rfrf) as a male parent, and carrying out paired cross to obtain F1-generation seeds; sowing the F1-generation seeds, and carrying out testcross on the material as the male parent and the F1-generation seeds to obtain fertile and sterile F2-generation seeds; sowing the F2-generation seeds, carrying out test cross on the material as the male parent and the sterile F2-generation seeds, retaining plants with target traits and harvesting F3-genration seeds; carrying out repeated two-generation test cross on the F3-generation seeds and the material as the male parent, retaining seeds with the target traits for each generation and obtaining developed sterile lines; taking the corresponding male parent material as a maintainer line andtaking a material with the genotype S(RfRf) or the genotype N / S(RfRf) as a restoring line. The method disclosed by the invention has the advantages that breeding procedures are simple, a breeding target is controllable, and three pepper cytoplasm male sterile line materials can be simultaneously bred on a large scale.

Description

technical field [0001] The invention relates to a plant selection and breeding method, in particular to a method for creating a capsicum cytoplasmic male sterile line and matching three lines simultaneously, belonging to the technical field of plant breeding. Background technique [0002] CMS refers to cytoplasmic male sterility (cytoplasmic male sterility) in crop breeding. Pepper cytoplasmic male sterile lines are controlled by cytoplasmic genes and nuclear genes respectively, wherein the cytoplasmic genes are respectively represented by N (fertile) and S ( sterility) and nuclear genes are represented by Rf (fertile) and rf (sterility) respectively. The first generation of hybrids prepared by using the capsicum cytoplasmic male sterile line has solved the problem of manual castration of inter-variety hybrid seed production. The pollination female parent of hybrid farming per mu is calculated on the basis of 3000 plants, and each plant pollinates 10 flowers, and a total of...

AI Technical Summary

Problems solved by technology

[0003] Although the pepper cytoplasmic male sterile line has been discovered since 1958, the method of obtaining the pepper cytoplasmic male sterile line from the restorer line as the female parent, the maintainer line as the male parent, and then selfing has not been reported.