Method for breeding rape temperature sensitive Polima cytoplasmic male sterile dual purpose line by microspore culturing process

Abstract

The invention relates to a breeding method for a rape Polima cytoplasmic male sterile line, in particular to a method for innovating the conventional Polima cytoplasmic male sterile line into a temperature sensitive Polima cytoplasmic male sterile dual purpose line directly by a microspore culturing process by selecting the Polima cytoplasmic male sterile line or the cytoplasmic male sterile lineof the same type, and belongs to the improvement on the breeding method for the rape Polima cytoplasmic male sterile system. In the method, the temperature sensitive Polima cytoplasmic male sterile dual purpose line which is partially fertile or partially sterile under the condition of fall seeding in Yangtze river basins and is sterile under the condition of summer seeding in a spring rape area is bred directly by adopting the Polima cytoplasmic male sterile line or the cytoplasmic male sterile line by the microspore culturing process.

Description

technical field [0001] The invention belongs to the improvement of the breeding method of the rape polima cytoplasmic male sterile line. Concretely, it is a method of selecting the Polyma cytoplasmic male sterile line and transforming the traditional sterile line into a thermosensitive dual-purpose line. Its international patent classification number is AO1H1 / 02. Background technique [0002] Researchers engaged in this technical field are well aware that in the past, the Polyma cytoplasmic male sterile line was bred to produce hybrid varieties through a three-line model, that is, the sterile line and the maintainer line were first crossed to breed the sterile line, and then the bred CMS line was bred. CMS lines are crossed with restorer lines to produce hybrid varieties. The CMS propagation method in the original three-line model needs to plant the CMS and the maintainer at the same time, and it is required to plant the CMS and the maintainer separately in proportion to p...

AI Technical Summary

Problems solved by technology

Therefore, not only the process is complicated and the labor load is large, but also it is difficult to ensure the purity of the breeding sterile line seeds.

The disadvantage of this traditional method is that the breeding procedure of the sterile line is relatively complicated, and the purity and uniformity of the reproduced sterile line are relatively poor.

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