Method for breeding rape temperature-sensitive polima cytoplasmic male sterile AB line in artificial auxiliary pollination selfing way
A male sterile line, artificially assisted technology, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of complicated breeding procedures, poor purity and uniformity of sterile lines, complicated procedures, etc., and achieve genetic The effect of high type homozygosity, high seed purity and simple process
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Embodiment 1
[0020] Embodiment 1: Breeding of thermosensitive polyma male sterile dual-purpose line TCMS986A
[0021] For the breeding process of thermosensitive Polyma cytoplasmic male sterile line 986A, see figure 1 . Under the condition of autumn sowing in Wuhan, in 2003, some male sterile single plants were selected from the nuclear + cytoplasmic male sterile line 986A at the initial flowering stage for self-pollination and self-fertilization. Some of the seeds were sown in Hezheng County, Gansu Province for the identification of sterility, and the remaining seeds were kept. In the autumn of 2003, under the conditions of summer sowing in Hezheng County, Gansu Province, the individual plants that were completely sterile were sown in autumn. In the spring of 2004, some sterile individual plants were selected at the early flowering stage for artificial pollination and self-fertilization. In May 2004, the self-fertilization single plants The seeds of the plant were divided into two parts...
Embodiment 2
[0022] Embodiment 2: Breeding of thermosensitive polyma male sterile dual-purpose line TCMS8086A
[0023] For the breeding process of the thermosensitive polyma cytoplasmic male sterile (TCMS) line TCMS8086A see figure 2 . Under the condition of autumn sowing in Wuhan, in 2003, some male sterile plants were selected from the nuclear + cytoplasmic male sterile line 8086A at the initial flowering stage for artificial assisted pollination and selfing. In May 2003, the seeds of the selfing single plant were divided into two, Some of the seeds were sown in Hezheng County, Gansu Province for the identification of sterility, and the remaining seeds were kept. In the autumn of 2003, under the conditions of summer sowing in Hezheng County, Gansu Province, the individual plants that were completely sterile were sown in autumn. In the spring of 2004, some sterile individual plants were selected at the early flowering stage for artificial pollination and self-fertilization. In May 2004,...
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