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Method for breeding rape temperature-sensitive polima cytoplasmic male sterile AB line in artificial auxiliary pollination selfing way

A male sterile line, artificially assisted technology, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of complicated breeding procedures, poor purity and uniformity of sterile lines, complicated procedures, etc., and achieve genetic The effect of high type homozygosity, high seed purity and simple process

Active Publication Date: 2013-04-10
武汉联农种业科技有限责任公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, not only the process is complicated and the labor load is large, but also it is difficult to ensure the purity of the breeding sterile line seeds.
The disadvantage of this traditional method is that the breeding procedure of the sterile line is relatively complicated, and the purity and uniformity of the reproduced sterile line are relatively poor.

Method used

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  • Method for breeding rape temperature-sensitive polima cytoplasmic male sterile AB line in artificial auxiliary pollination selfing way
  • Method for breeding rape temperature-sensitive polima cytoplasmic male sterile AB line in artificial auxiliary pollination selfing way

Examples

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Effect test

Embodiment 1

[0020] Embodiment 1: Breeding of thermosensitive polyma male sterile dual-purpose line TCMS986A

[0021] For the breeding process of thermosensitive Polyma cytoplasmic male sterile line 986A, see figure 1 . Under the condition of autumn sowing in Wuhan, in 2003, some male sterile single plants were selected from the nuclear + cytoplasmic male sterile line 986A at the initial flowering stage for self-pollination and self-fertilization. Some of the seeds were sown in Hezheng County, Gansu Province for the identification of sterility, and the remaining seeds were kept. In the autumn of 2003, under the conditions of summer sowing in Hezheng County, Gansu Province, the individual plants that were completely sterile were sown in autumn. In the spring of 2004, some sterile individual plants were selected at the early flowering stage for artificial pollination and self-fertilization. In May 2004, the self-fertilization single plants The seeds of the plant were divided into two parts...

Embodiment 2

[0022] Embodiment 2: Breeding of thermosensitive polyma male sterile dual-purpose line TCMS8086A

[0023] For the breeding process of the thermosensitive polyma cytoplasmic male sterile (TCMS) line TCMS8086A see figure 2 . Under the condition of autumn sowing in Wuhan, in 2003, some male sterile plants were selected from the nuclear + cytoplasmic male sterile line 8086A at the initial flowering stage for artificial assisted pollination and selfing. In May 2003, the seeds of the selfing single plant were divided into two, Some of the seeds were sown in Hezheng County, Gansu Province for the identification of sterility, and the remaining seeds were kept. In the autumn of 2003, under the conditions of summer sowing in Hezheng County, Gansu Province, the individual plants that were completely sterile were sown in autumn. In the spring of 2004, some sterile individual plants were selected at the early flowering stage for artificial pollination and self-fertilization. In May 2004,...

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Abstract

The invention relates to a breeding method of a rape polima cytoplasmic male sterile line and belongs to the improvement of a breeding method of a rape polima cytoplasmic male sterile line. Particularly, the polima or the same type cytoplasmic male sterile line is selected, and the artificial auxiliary pollination selfing way is used for innovating the traditional polima cytoplasmic male sterile line to a temperature-sensitive polima cytoplasmic male sterile AB line. The polima cytoplasmic or the same type cytoplasmic male sterile line is firstly adopted, and the temperature-sensitive polima cytoplasmic male sterile AB line which represents partial fertility or partial sterility under autumn sowing conditions in a Yangtze river valley and represents sterility during summer sowing in a spring rape production area is bred from the sterile line through the artificial auxiliary pollination selfing breeding way.

Description

technical field [0001] The invention belongs to the improvement of the breeding method of the rape polima cytoplasmic male sterile line. Specifically, it is a method of selecting the Polyma cytoplasmic male sterile line and transforming the traditional sterile line into a thermosensitive dual-purpose line. Its international patent classification number is AO1H1 / 02. Background technique [0002] Researchers engaged in this technical field are well aware that in the past, the Polyma cytoplasmic male sterile line was bred to produce hybrid varieties through a three-line model, that is, the sterile line and the maintainer line were first crossed to breed the sterile line, and then the bred CMS line was bred. CMS lines are crossed with restorer lines to produce hybrid varieties. The CMS propagation method in this three-line model needs to plant the CMS and the maintainer at the same time, and it is required to plant the CMS and the maintainer separately in proportion to avoid c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 杨光圣
Owner 武汉联农种业科技有限责任公司
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