IgG subtype typing detection reagent card, and preparation method thereof

A detection reagent and typing technology, which is applied in the field of IgG subtype detection reagent card and its preparation, can solve the problems of long inspection time, inability to detect blood type-specific IgG subtypes, limited reference value, etc., and achieve a wide range of applications , preventing hemolytic disease of newborns and improving accuracy

Inactive Publication Date: 2015-11-18
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ELISA detection reagent of Sigma Company in the United States can detect the total IgG subclasses in the blood, but it cannot detect the blood group-specific IgG subclasses. It has limited reference value for clinical diagnosis and treatment, and the method takes a long time to check and is not suitable for single samples and emergency departments. instant detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1, preparation of gel suspension medium

[0029] Described gel suspending medium formula is as follows:

[0030] Methylparaben (5.5-6.5) x10 -4 g / ml

[0031] Propyl p-hydroxybenzoate (1.0-1.5) x10 -4 g / ml

[0032] Glycine (1.6-1.9) x10 -2 g / ml

[0033] Sodium chloride (1.7-1.8) x10 -3 g / ml

[0034] Potassium dihydrogen phosphate (2.1-2.4) x10 -4 g / ml

[0035] Disodium hydrogen phosphate (4.6-4.8) x10 -4 g / ml

[0036] The above reagents were dissolved in distilled water, and the final pH value was 6.6-6.8;

[0037] Step 2. Screening of polyacrylamide dextran gel

[0038] Select polyacrylamide dextran gel, the gel particle size is 30-60 nanometers, soak with the gel suspension medium prepared in step 1, and then wash with the gel suspension medium for 3-5 times to remove damaged gel fragments And aggregated gel particles to obtain uniform, complete and spherical gels;

[0039] Step 3. Antibody selection

[0040] Choose a monoclonal anti-IgG1 antibody w...

Embodiment 2

[0060] The method of using the IgG subtype detection reagent card is as follows:

[0061] 1. The eight micro-column gel tubes on the IgG subtype detection reagent card are two tubes containing anti-IgG1 antibody, two tubes containing anti-IgG2 antibody, and two tubes containing anti-IgG3 antibody. Gel tube and two gel tubes containing anti-IgG4 antibody;

[0062] 2. Use physiological saline to adjust the red blood cells of the newborn to be tested to a volume percentage of 0.8-1%, and then absorb 50 U1 of the adjusted red blood cells and add them to eight microcolumn gel tubes;

[0063] 3. Place the IgG subtype detection reagent card in a special centrifuge, first centrifuge at 900rpm for 2 minutes, then centrifuge at 1500rpm for 3 minutes, take it out and visually judge the result and record it;

[0064] 4. Result judgment

[0065] Positive result: red blood cells floating on the surface of the gel or in the gel is a positive reaction;

[0066] Negative result: red blood c...

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PUM

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Abstract

The invention relates to an IgG subtype typing detection reagent card, and a preparation method thereof. The preparation method comprises following steps: gel suspension medium preparation; polyacrylamide dextrangel screening; antibody selection; and preparation, packaging, and sealed preservation of a gel containing IgG antibodies. Application range of the IgG subtype typing detection reagent card and the preparation method is wide; sensitivity, specificity, and stability are high; and the IgG subtype typing detection reagent card possesses significant importance on in-time analysis, determination, and intervention treatment of hemolysis disease of fetus and newborn.

Description

technical field [0001] The invention relates to an IgG subtype detection reagent card and a preparation method thereof. Background technique [0002] Immunoglobulin (IgG) is the main molecule of human immunity, accounting for 75% of the total immunoglobulin in the plasma of healthy people. When a certain pathogen invades the human body, its surface antigen can be used as a substance to stimulate the human immune system , so that the body can produce specific immunity to it, which comes from antibodies produced in the body. Since a decrease in the level of one IgG subtype can be accompanied by an increase in the level of one or several other IgG subtypes, the total IgG level remains normal. Therefore, even if the total amount of IgG is in the normal range or slightly lower than the normal range, it is necessary to measure the IgG subtype level. [0003] During the long pregnancy, due to the reality of fetal-maternal hemorrhage, a small amount of fetal blood enters the mothe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 陈玉平孙兵钱国强
Owner JIANGYIN LIBO MEDICINE BIOTECH
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