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Preparation method of low molecular weight konjac oligosaccharide for regulating intestinal flora

A technology for regulating intestinal flora and konjac oligosaccharides, which is applied in the field of konjac oligosaccharides, can solve the problems of konjac functional components research and application limitations, low specificity, high cost, etc., and achieve environmental protection, simple preparation, low cost effect

Active Publication Date: 2015-11-18
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, konjac oligosaccharides are obtained by acid hydrolysis and enzymatic hydrolysis. The former has low specificity, and the process involves acid and alkali, which is easy to cause environmental pollution.
In view of the shortcomings of the wide molecular weight distribution of oligosaccharides obtained by enzymatic hydrolysis, many reports use ultrafiltration and one-step alcohol precipitation to fractionate the obtained oligosaccharides, but the former faces high costs in actual industrial production (industrial ultra- filter equipment purchase costs at least 100,000 or more), the disadvantage of low yield, although the latter one-step alcohol precipitation method is simple to prepare and suitable for large-scale production, but monosaccharides are often enriched in the product, and the removal of monosaccharides requires New production process, these problems make the research and application of konjac functional components limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1. The process of konjac mannan producing specific molecular weight konjac oligosaccharides, the specific steps are as follows:

[0012] (1) Konjac mannan was hydrolyzed by β-mannanase to obtain konjac oligomannose hydrolyzate, as follows: the concentration of konjac mannan was 2%, the pH value was adjusted to 7.0, and the temperature was 35°C. The concentration ratio of the substrate is 500U / g, and the enzymatic hydrolysis is hydrated for 6h. .

[0013] (2) Konjac mannose oligosaccharide hydrolyzate is deactivated at 100°C, concentrated to one-tenth of the original volume (about 100mL) by a rotary evaporator, and 900mL of pure ethanol is added to the hydrolyzate, stirred overnight, after precipitation, centrifuged, Take the supernatant to obtain the first separation liquid.

[0014] (3) Concentrate the separation liquid from the first step by rotary evaporation to one-tenth of its original volume (about 100mL), then add 200mL double volume of acetone, after precipita...

Embodiment 2

[0015] Example 2 The present invention studies the mouse experiment on the activity of regulating the intestinal flora of the prepared mannose oligosaccharides

[0016] In this experiment, the effects of mannose oligosaccharides on the intestinal morphology and intestinal flora of normal mice were investigated. The specific method is: take 50 mice, randomly divide them into four experimental groups and a control group, and use different doses (1g / kg·d, 2g / kg·d, 4g / kg·d, 8g / kg· d) The mannose oligosaccharide solution and an equal volume of normal saline were given to the stomach for 30 days, and the effects of different additions on the intestinal morphology, short-chain fatty acids (SCFA) and microbial flora of the cecal contents of mice were observed and compared. It was found that when the dosage reached 4g / kg·d, the total acid content increased to 103.35±10.08μmol / g, which was significantly increased (p<0.05); when the added amount was as high as 8g / kg·d , the total acid c...

Embodiment 3

[0018] The difference between the products prepared by different methods:

[0019] method one step alcohol precipitation One-step acetone treatment One-step alcohol precipitation and two-step acetone treatment Monosaccharide content >10% >10% <2%

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Abstract

The invention relates to konjac oligosaccharide for regulating intestinal flora, in particular to a novel method, namely a method to acquire konjac oligosaccharide mannose mixture with low molecular weight, narrow distribution, low monosaccharide content and higher purity, by means of primary alcohol precipitation and secondary acetone treatment after enzymatic hydrolysis. The method includes the steps of hydrolyzing konjac mannan by beta- mannanase to obtain konjac mannose oligomer hydrolysate, adding ethanol precipitation of certain volume into the konjac mannose oligomer hydrolysate concentrated, concentrating supernate, adding acetone of certain volume, performing centrifuging after precipitating, removing supernate, and collecting precipitation to obtain the oligosaccharide mixture mainly containing konjac mannobiose, mannotriose, mannotetraose, mannopentaose, mannohexaose and mannoheptaose. The konjac oligosaccharide prepared by the method has a certain effect in regulating the intestinal flora.

Description

technical field [0001] The invention relates to a preparation method of konjac oligosaccharides capable of regulating intestinal flora, specifically, the invention relates to a konjac with low molecular weight, narrow molecular weight, environmentally friendly process and low cost, which has the function of regulating intestinal microbial community structure Oligosaccharides. Background technique [0002] The human gastrointestinal tract is an extremely complex micro-ecological system, which hosts a large number and variety of microorganisms. These microorganisms participate in a series of life activities such as metabolism and immunity of the human body to maintain the health of the body. Once the balance of this system is broken, it will lead to the imbalance of intestinal flora, which will cause or even aggravate many diseases. In recent years, with the development of society and economy, great changes have taken place in the dietary structure of Chinese residents, espe...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04
Inventor 蒋敏史劲松许正宏马延和李恒罗春勤
Owner JIANGNAN UNIV
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