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Rhizobium and application thereof

A rhizobia and seed technology, applied in the direction of application, bacteria, fertilizer mixture, etc., can solve the problems of high saline-alkali content and lack of fertilizer sources in saline-alkali land, and achieve the effect of improving saline-alkali land, significant growth-promoting effect, and great application and promotion value

Active Publication Date: 2015-11-18
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Tianjing is often used to improve saline-alkali land, and saline-alkali land not only has high saline-alkali content, but also faces a lack of fertilizer sources

Method used

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  • Rhizobium and application thereof
  • Rhizobium and application thereof
  • Rhizobium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: the acquisition of rhizobia

[0024] The root nodules were collected from the salinized soil of the Yellow River Delta, which grew vigorously above and below the ground, and the nodules were collected. After the surface of the nodules was sterilized, the nodules were smashed, and the leachate was spread on a YMA plate, and cultured upside down at 28°C for 3 years. -5 days, when a round colony with neat edges, milky white translucence, and more extracellular polysaccharides grows, pick a single colony and streak repeatedly until it is purified (see figure 1 ).

[0025] The formula of YMA solid medium (yeast extract mannitol agar medium) is: mannitol 10g / L, yeast extract 3g / L, K 2 HPO 4 0.25g / L, KH 2 PO 4 0.25g / L, MgSO 4 0.2g / L, CaCO 3 3g / L, NaCl 0.1g / L, dilute to 1000mL with deionized water, and divide them into Erlenmeyer flasks, and add agar powder with a final concentration of 20g / L to each bottle.

[0026] 1) Identification of rhizobia

[0027] ...

Embodiment 2

[0039] The bacterial agent preparation application of embodiment 2 bacterial strain YIC4027

[0040] 1) Strain activation

[0041] Pick up the YIC4027 colony with a sterilized inoculation loop, streak it on the YMA solid medium, and culture it upside down at 28°C for 2-3 days until the colony grows well.

[0042] 2) Expansion of cultivation

[0043] Use a sterilized inoculation loop to pick one to two rings of bacteria, inoculate into 200mL sterilized liquid YMA liquid medium, shake and culture at 28°C and 160 rpm for 24 to 48 hours, and cultivate until OD600≥1.5, That is, the bacteria agent to be used. Put the bacterial agent in a refrigerator at 4°C and it can be stored for 1 week.

[0044] 3) Application of Rhizobium

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Abstract

The invention relates to a technology for improving a saline and alkaline land and particularly relates to rhizobium and an application thereof. The rhizobium (Ensifersp.) YIC4027 has been collected in China General Microbiological Culture Collection Center (short for CGMCC and the address is No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing), and the collection number is CGMCC No. CGMCC 10994. The rhizobium has the advantages that the strain growth speed is fast, saline and alkaline resistance is achieved, effective symbiotic nitrogen fixation with sesbania can be achieved, the growth of the sesbania can be obviously promoted under the environment of greenhouses and large fields, the yield of above-ground parts and under-ground parts of plants can be obviously increased, and the yield and the quality of the sesbania are improved; the benefit for planting the sesbania to improve the saline and alkaline lands and other barren lands is achieved, the use of fertilizer is reduced, the environmental pollution caused by the fertilizer is reduced and finally the purposes of improving the saline and alkaline soil and barren soil, promoting ecological restoration and protecting the ecological environment are achieved.

Description

technical field [0001] The invention relates to a technique for improving saline-alkali land, in particular to a rhizobia and its application. Background technique [0002] Rhizobia are a group of Gram-negative bacteria that form root nodules or stem nodules in leguminous plants for symbiotic nitrogen fixation. When the rhizobia symbioses with the matching leguminous plants, they form an effective symbiotic tissue-root nodule or stem nodule. In the root nodule or stem nodule, the rhizobia converts nitrogen in the air into ammonia, which is provided to the host plant as a nitrogen source, and Leguminous plants provide carbon sources and nutrients for rhizobia, and the two form a good mutualistic symbiotic relationship. [0003] Since the interaction between rhizobia and leguminous plants needs to undergo a series of interactive molecular dialogues, and this symbiotic process will be affected by environmental factors, the indigenous highly efficient nitrogen-fixing rhizobia w...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/04C05G3/80
Inventor 李岩解志红李项岳任承钢刘卫吴海龙姜南
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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