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A kind of method for rapid propagation of tamarisk tissue culture

A technology of tissue culture and culture medium formula, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as inability to apply production, bottlenecks in rapid propagation of Tamarix tissues, etc., and achieve large-scale production and improve reproductive efficiency Effect

Inactive Publication Date: 2017-06-27
QINGDAO ZHENGJIE INDAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the above prior art tamarix tissue culture rapid propagation bottleneck, the problem that cannot be applied to production, the invention provides a kind of method for rapid propagation of tamarix tissue culture

Method used

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  • A kind of method for rapid propagation of tamarisk tissue culture
  • A kind of method for rapid propagation of tamarisk tissue culture
  • A kind of method for rapid propagation of tamarisk tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The selection of embodiment 1 primary substratum

[0035] 【1】Materials: Tamarix explants are taken from the fresh shoots that have just grown in the field. After the materials are collected, fresh shoots are selected as test materials.

[0036] 【2】Pretreatment of test materials: Soak in distilled water for 0.5h, add a small amount of washing powder and two drops of Tween during soaking, then rinse with tap water for 2h, after rinsing, put the explants on the inoculation table for inoculation,

[0037] 【3】Preparation before inoculation: Before inoculation, wipe the inoculation table with 75% alcohol, and turn on the ultraviolet light for 0.5h. The Tamarix explants were washed 3 times with sterile water, then soaked in 75% alcohol for 30 seconds, and then washed 3 times with sterile water.

[0038] [4] Sodium hypochlorite sterilization: sterilize with 2% sodium hypochlorite for 3 minutes, 5 minutes, 7 minutes, 9 minutes, and then connect to various media based on MS medium...

Embodiment 2

[0043] Embodiment 2: the selection of subculture medium

[0044] After the aseptic explants were obtained, they were subcultured on the subculture medium (see Table 2), and the seedlings were transferred once every 20 days. There were differences in the differentiation of tamarisk willow buds, the length of internodes of branches, and the growth potential, as shown in Table 2.

[0045] Table 2 The performance of tamarisk aseptic seedlings on the subculture medium

[0046]

[0047] Conclusion: In subculture, the E medium is MS+zeatin ZT (0.5mg / L)+IBA(0.1 mg / L)+sucrose (30g / L)+agar (7g / L) is more suitable for the growth and differentiation of Tamarix . The subculture medium is MS+zeatin ZT (0.5mg / L)+IBA(0.1mg / L)++sucrose (30g / L)+agar (7g / L).

Embodiment 3

[0048] Embodiment 3: the selection of rooting medium

[0049] The key to tissue culture of Tamarix is ​​to find out the rooting formula. In the rooting culture, the rooting formula based on MS and 1 / 2MS medium is used. There are great differences in the rooting conditions of the main root and lateral root, as shown in Table 3. Rooting with P medium 1\2MS+IBA(0.5mg / L)+NAA(0.1mg / L)+GA(0.1mg / L)+sucrose(15g / L)+agar(7g / L), pH=5.8 best effect.

[0050] Table 3 Root culture of Tamarix

[0051]

[0052] In summary, it can be concluded that the complete formula of tamarisk tissue culture is as follows:

[0053] First generation: MS+6-BA(1.0mg / L)+IBA(0.1 mg / L)+GA(0.1 mg / L)+sucrose (30g / L)+agar (7g / L), pH=5.8

[0054] Subculture: MS+(0.5mg / L)+IBA(0.1 mg / L)+ +sucrose (30g / L)+agar (7g / L), pH=5.8

[0055] Rooting: 1\2MS + indole butyric acid IBA (0.5mg / L) + NAA (0.1 mg / L) + GA (0.1 mg / L) + sucrose (15g / L) + agar (7g / L), pH=5.8

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Abstract

The invention relates to the technical field of wood tissue culture seedling raising in forest science, in particular to a Tamarix chinensis tissue culture and rapid propagation method. The method includes: acquisition of aseptic seedlings, with an involved medium containing 6-BA, IBA, GA, sucrose and agar; subculture: inoculating the aseptic seedlings into a subculture medium to conduct culture so as to obtain clustered shoots, with the medium containing ZT, IBA, sucrose and agar; rooting culture: inoculating the clustered shoots into a rooting medium containing IBA, NAA, GA, sucrose and agar to obtain rooted tissue cultured seedlings; and acclimatization and transplantation. The propagation method has high propagation coefficient and short propagation cycle, the obtained seedlings are strong and have a lot of root systems, and the survival rate of transplantation is over 96%. The method provided by the invention omits the strong seedling culture step in conventional methods, improves propagation efficiency, makes the Tamarix chinensis tissue culture and rapid propagation technology move towards production from laboratories, realizes large-scale production of Tamarix chinensis tissue culture seedlings, and provides technical support for promotion of Tamarix chinensis rapid propagation.

Description

technical field [0001] The invention relates to the technical field of tree tissue culture and seedling cultivation in forestry science, and relates to a method for rapid propagation of woody plant tissue culture, in particular to a method for rapid propagation of tamarix tissue culture. Background technique [0002] Tamarix chinensis Lour., also known as Tamarix chinensis Lour., is a typical perennial saline-secreting woody halophyte in the genus Tamarix L. of the family Tamaricaceae. Humid saline and sandy wastelands. Its branches, leaves, and flowers have very high ornamental value, and have the characteristics of drought resistance, salt and alkali resistance, and water and humidity resistance (Zhang Shengli et al., 2015). Saline-alkali land improvement and afforestation tree species are also recognized as the number one salt-tolerant tree species among afforestation tree species in my country. The plants can withstand 2.4% salt water irrigation and can grow normally on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 袁正杰袁迅道王衍军聂琛董俊祝解孝满鲁仪增
Owner QINGDAO ZHENGJIE INDAL
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