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Grading extraction method of humic acid in frond

A technology for fractional extraction and humic acid, applied in the field of separation and extraction of humic acid

Inactive Publication Date: 2015-11-11
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fractionation of algal humic acid has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Fractional extraction of humic acid in Jiaozhou Bay brown algae sample:

[0045] In June 2014, samples of brown algae from Jiaozhou Bay were collected;

[0046] XAD-8 resin column; Sigma company;

[0047] Sodium hydroxide: analytically pure; hydrochloric acid: analytically pure;

[0048] The concrete operation of this embodiment is as follows:

[0049] Step a, algae body pretreatment: Take the air-dried algae to remove impurities, dry and grind at 40°C and pass through a 40-mesh sieve to obtain 4Kg of algae powder;

[0050] Step b, algae Soxhlet extraction:

[0051] Put 4Kg of algae powder in a Soxhlet extractor, and use ether, acetone, 95% ethanol, dioxane and deionized water as extraction liquids for Soxhlet extraction, and finally obtain 3.45Kg of solids, marked as Soxhlet. Extract algae; in the process of extracting, at first adopt ether as the extracting solution for extracting, every 3-5h, measure the absorbance of the extracting solution unde...

Embodiment 2

[0079]Example 2: The difference between this example and Example 1 is that in addition to all the operating steps in Example 1, this example also includes the following operations: In the step g, each part of fulvic acid solution is obtained by centrifugation , then use the dissolved organic carbon measuring instrument to measure the dissolved organic carbon content of the obtained fulvic acid solution. If the measured dissolved organic carbon TOC>5mg / L, then the solid-state purified humic acid solution corresponding to the centrifuged fulvic acid solution The acid subcomponent repeats the operation of adding acid, stirring and standing still in step g until TOC<5 mg / L, and then proceeds to step h.

Embodiment 3

[0080] Embodiment 3: The difference between this embodiment and Example 1 is that in this embodiment, in addition to all the operating steps of Example 1, step i is also included: adding the humic acid subcomponent 1 of the algae body to the algae body After the humic acid subcomponent 6 is dried at 80-100°C for 24 hours, the ash content obtained is measured; if the ash content of a part of the humic acid subcomponent of the algal body is greater than 0.1% by dry weight, the algal The humic acid subcomponents of the body are dissolved with 0.1mol / L sodium hydroxide under the condition of nitrogen protection, and deionized water is added to make the concentration of humic acid in the solution = 1-3g / L, then the pH is adjusted to 5-9, and then added Concentrated hydrochloric acid, concentrated hydrofluoric acid and deionized water, so that the solid-to-liquid ratio is 1:10, the concentration of hydrochloric acid is 0.1mol / L, and the concentration of hydrofluoric acid is 0.3mol / L....

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Abstract

The invention discloses a grading extraction method of humic acid in frond. The grading extraction method includes the following steps that frond preprocessing, soxhlet extraction of the frond, calcium chloride and acid pickling of the frond, sodium carbonate processing of the frond, alkali liquor extraction of the frond, silicon removal of frond humic acid, fulvic acid removal of frond humic acid and desalting and solidifying of frond humic acid. By means of the mode, the humic acid can be extracted from the frond, subfraction separation is conducted through an XAD-8 resin separation technology, and a graded frond humic acid subfraction is finally obtained.

Description

technical field [0001] The invention relates to humic acid separation and extraction technology, in particular to a method for fractional extraction of humic acid in algal bodies. Background technique [0002] Humic acid is a macromolecular organic compound widely present in environmental media such as soil, water, and sediment. Humic acid is soluble in alkaline and neutral solution, but insoluble in acid. Humic acid contains a large number of active functional groups such as carboxyl, phenolic hydroxyl, carbonyl, amino and mercapto, so it has high reactivity. For example, in the natural environment, it can interact with toxic heavy metal ions and organic pollutants (such as pesticides, PPCPs and PAHs) in the environment to form chemically and biologically stable water-soluble and water-insoluble substances, thereby changing its Migration, transformation patterns and bioavailability. [0003] It is generally believed that humic acid is formed by animals and plants and the...

Claims

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Application Information

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IPC IPC(8): C08H99/00
Inventor 吴丰昌白英臣郭飞宋凡浩陈曲
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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