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Method used for extracting burdock fructose oligosaccharides from burdock root

A technique for burdock oligofructose and burdock root is applied in the field of extracting burdock oligofructose, which can solve the problems of low protein removal rate, large polysaccharide loss rate, and difficulty in removing organic solvents completely, and achieves maintaining physical and chemical properties and uniform molecular weight. , the effect of difficult target product denaturation

Active Publication Date: 2015-11-11
博德生物技术(德州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these methods, except the papain method, organic solvents are used, which are difficult to completely remove after deproteinization
Although the papain method is safe, it has the disadvantages of low protein removal rate and excessive polysaccharide loss rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1,000g of fresh burdock root after cleaning and peeling, and beat the burdock root with a beater; add 2,500mL of pure water after beating, heat to 80°C, and perform homogenization during the heating process; after homogenization, use a peristaltic The pump was fed into the high-voltage pulsed electric field extraction equipment at 100mL / min for extraction, and the parameters were set to the electric field strength of 10kV / cm, and the number of pulses was 6; after the high-voltage pulsed electric field was extracted, it was centrifuged, and the supernatant was taken; Membrane filtration, discard the upper part of the membrane; the lower part of the membrane obtained by ultrafiltration is filtered using an ultrafiltration membrane with a molecular weight cut-off of 800Da until the density of the upper part of the membrane is 1.11g / mL, and the upper part of the membrane is collected; spray-dried to obtain burdock fructooligosaccharide 53.6g.

[0032] Carry out chemica...

Embodiment 2

[0034] Take 100g of dried burdock root slices, crush them through a 100-mesh sieve with a pulverizer, add 1,500mL of pure water, heat to 80°C, and perform homogenization during the heating process; after homogenization, use a peristaltic pump to feed the The high-voltage pulse electric field extraction equipment is used for extraction, and the parameters are set to electric field strength 20kV / cm, pulse number 5; after the high-voltage pulse electric field extraction is completed, centrifuge and take the supernatant; filter with an ultrafiltration membrane with a molecular weight cut-off of 7,000Da, and discard the membrane Upper part; the lower part of the membrane obtained by ultrafiltration is filtered using an ultrafiltration membrane with a molecular weight cut-off of 1,000Da until the density of the upper part of the membrane is 1.08g / mL, and the upper part of the membrane is collected; spray-dried to obtain 26.4g of burdock fructooligosaccharide.

[0035]Carry out chemic...

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Abstract

The invention provides a method used for extracting burdock fructose oligosaccharides from burdock root. The method mainly comprises following steps: processed burdock root is subjected to high intensity pulsed electric field extraction; an obtained extract is subjected to centrifugation precipitation so as to obtain a supernate; the supernate is subjected to secondary ultrafiltration membrane filtration so as to obtain a burdock fructose oligosaccharide concentrated solution; and the burdock fructose oligosaccharide concentrated solution is subjected to drying so as to obtain burdock fructose oligosaccharides with uniform molecular weight.

Description

technical field [0001] The invention relates to a method for extracting natural products from plants, in particular to a method for extracting burdock fructooligosaccharides from burdock roots. Background technique [0002] Burdock root is the root of burdock (Arctium lappa L.), a plant of Compositae, and burdock is a better medicine and food homologous plant. Burdock root is rich in oligosaccharides, called burdock fructooligosaccharides, burdock oligosaccharides, burdock chrysanthemum sugar etc. According to reports, burdock fructooligosaccharides have the function of effectively lowering blood lipids and are a valuable natural product. [0003] It is known that there are many methods for the extraction of burdock fructooligosaccharides in burdock roots, such as direct water extraction (Hao Linhua, etc., Journal of Ocean University of China, May, 2004, the 3rd phase of volume 34), microwave extraction (Wang Hongxin, etc., Chinese patent application number 200910182291.X)...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张硕陈学文
Owner 博德生物技术(德州)有限公司
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