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Method for improving rooting of vaccinium ashei tissue culture seedlings

A technology for rabbit-eye blueberry and tissue culture seedlings, which is applied in the field of plant tissue culture, can solve the problems of hard to see, difficulty in rooting of rabbit-eye blueberry tissue culture seedlings, difficulty in rooting of tissue culture seedlings, etc., and achieves robust seedlings, simple method and effect. significant effect

Active Publication Date: 2015-11-11
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Highbush blueberries suitable for planting in the north mostly use tissue culture to cultivate seedlings, while rabbit-eye blueberries basically rely on summer twig cuttings to raise seedlings. Although there are some reports that tissue culture methods can be used to obtain rabbit-eye blueberry seedlings, there are few in the market. It is difficult to see, and the reason is that it is difficult to root the rabbit-eye blueberry tissue culture seedlings in the conventional tissue culture method
In order to overcome the difficulty in rooting of rabbit-eye blueberry tissue culture seedlings, many scholars have carried out some research, but there is no detailed report on the rooting matrix of its tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: The effects of different hormones on rabbit eyes blueberry group Pei Miao root

[0016] Choose the strong group cultivation of the secondary cultivation twice, and inoculate to the 1 / 2WPM medium with different types and concentration hormones (NAA, IBA, 2,4-D and NAA, IBA) with agarRooting.For each vaccination, repeat 3 times, observe and count the root cause of the test tube seedlings after 45 days.

[0017] It can be seen from Table 1 that in the medium that adds different concentration hormones, the rooting rate and average root length of the custody of the medium containing NAA medium are significantly higher than other hormones.MG / L) The rooting effect is better than the high concentration (0.5mg / L, 1.0mg / L).The effect of IBA -induced rooting is second. When IBA is rooted in the induced hormone, the high concentration (0.75mg / L, 1.0mg / L) is better.IAA is not suitable as a hormone for blueberries, because some plants are found to die in the experiment for a p...

Embodiment 2

[0020] Example 2: The impact of different cultivation substrates on rabbit eyes blueberry group Pei Miao root

[0021] Choose the strong group cultivation of the secondary cultivation twice, and inoculate to different materials (agar, vermiculite, river sand, peat soil, peat soil, and vermiculite mixed with 1: 1) as the matrix, add 0.2mg / / add 0.2 mg / / LNAA's 1 / 2WPM medium induces rooting.30 plants were vaccinated in each substrate, repeated 3 times, and observed and statistical the root cause of the test tube seedlings after 45 days.

[0022] It can be seen from Table 2 that when the vermiculite is used as a substrate, the rooting effect of the blueberry group cultivation is the best. The rooting rate is 97.4%, which is significantly higher than the other matrix.Pei Miao plant is strong.The rooting rate of breeding seedlings in the agar is lower than vermiculite, and the roots are slightly wounded.It was found in the experiment that the plants with peat soil and river sand were we...

Embodiment 3

[0025] Example 3: Screening of the best root conditions

[0026] Choose the strong group cultivation of the secondary cultivation twice, and inoculate to the 1 / 2WPM medium with different types and concentration hormones (NAA, IBA, 2,4-D and NAA, IBA) in different types of vermiculite and adding different types and concentrationsIntrailed.For each vaccination, repeat 3 times, observe and count the root cause of the test tube seedlings after 45 days.

[0027] It can be seen from Table 3 that in the medium with a concentration of NAA 0.2mg / L, the rooting effect of the blueberry group cultivation is the best, with the highest root rate, 97.2%, and the most rooted., Far better than other treatment.When IBA0.75mg / L is added, the root effect of blueberries is also better, second only to NAA0.2mg / L. The rooting rate is 87.5%, and the average rooting number is 18.8.And it is found that the treatment of unpaid hormones (treatment 1: only remove ionic water) can also be 40.7%, and the number...

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Abstract

The invention relates to a method for improving rooting of vaccinium ashei tissue culture seedlings. The method is characterized in that high-quality vaccinium ashei adventitious buds obtained by primary culture and subculture proliferation are used as materials, vermiculites are used as substrates, and the materials are induced on a 1 / 2 WPM culture medium added with suitable-concentration NAA to take roots, wherein the materials are induced and cultured for 40-60 days and the rooting rate can reach more than 95%; and the rooting quantity of a single plant is more than 20, the length of each root is 2.0cm-3.0cm and rooted seedlings grow healthy and strong, the transplanting survival rate is high and the recovery period is short. The rooting substrates which save cost and have high efficiency are selected and conventional tissue culture conditions are adopted, so that the rooting rate of the vaccinium ashei tissue culture seedlings is improved by 34.7% and the transplanting survival rate is improved by 60.3%; the problems in current production that the rooting rate of the vaccinium ashei tissue culture seedlings is low, the transplanting is difficult and the like are solved; and a high-quality efficient propagation method is provided for producing high-quality variety germchits of vaccinium ashei and reference is provided for other types of blueberry tissue culture seedlings.

Description

Technical field [0001] The present invention involves the field of plant tissue training technology, and specifically to improve the root rate of rabbit eyes blueberry group. Background technique [0002] blueberry( Vaccinium SPP.) The more rhododendron (Ericaceae) is a perennial deciduous or evergreen shrub fruit tree, also known as blue pulp or more orange.As an important member of the third -generation emerging small fruit trees, blueberries are loved by consumers at home and abroad. In recent years, it has developed rapidly in my country in recent years.Rabbit eye blueberries are the main types of blueberries grown in southern my country. They are currently cultivated in the central and southern regions of my country in Jiangsu, Zhejiang, Jiangxi, and Guizhou.Most of the high -clump blueberries suitable for the north are mostly cultivated, and the rabbit eye blueberries basically rely on summer tender cuttings and seedlings. Although there are also some reports that they can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 吴文龙王小敏胡淑英闾连飞李维林
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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