A strain of bacteriophage that can enter the blood through oral administration and its application
A bacteriophage and monomer technology, applied in the field of bacteriophage, can solve the problems of polluting bedding, increasing broilers, and economic losses in the breeding industry, and achieve the effect of pollution control
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[0013] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not used to limit the present invention.
[0014] A. Screening and purification of phage
[0015] (1) Collection of samples
[0016] The chicken manure samples in the present invention were collected from a commercial chicken farm in Gaomi, Weifang City, Shandong Province.
[0017] (2) Specific amplification of coliphages in samples
[0018] Take 2g of the sample and add it to the pre-amplified Escherichia coli (the host bacteria addition ratio is 1:10), and place it on a constant temperature shaker at 37°C, 200r / min for 6-8h.
[0019] (3) Double-layer plate test to detect the presence of specific phages
[0020] Autoclave the nutrient agar solid medium with agar co...
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