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Method of two-step united multi-strain fermented feed

A combined fermentation and multi-strain technology, applied in animal feed, animal feed, application, etc., can solve the problems of low total number of probiotic colonies, limited growth of aerobic bacteria, and insignificant increase in protein content, etc., to achieve good growth and reproduction, The effect of increasing content

Active Publication Date: 2015-10-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with fruit and vegetable dregs, soybean whey liquid, canteen swill, silage, etc., the solid fermented feed with corn flour and palm meal as the main raw materials has more stable product quality, is more suitable for storage and transportation, and is conducive to commercialization Large-scale production mainly includes the following production methods: (1) only the fermentation method in the anaerobic fermentation stage, after the strains are mixed with the feed, they are directly sealed in bags for anaerobic cultivation. , the growth of aerobic bacteria is limited, resulting in low hydrolytic enzyme activity in raw materials, which cannot hydrolyze macromolecular substances in feed into small molecular substances, and thus cannot provide sufficient nutrients for anaerobic fermentation probiotics
The protein content in the final feed was not significantly increased, the hydrolytic enzyme activity was low, and the total number of probiotic colonies was low

Method used

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  • Method of two-step united multi-strain fermented feed
  • Method of two-step united multi-strain fermented feed
  • Method of two-step united multi-strain fermented feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 two strains aerobic, anaerobic two-step fermented feed

[0042] The specific implementation steps are as follows:

[0043] A. Cultivation of seeds

[0044] a. Preparation of pure culture of Bacillus subtilis seeds: transfer the activated Bacillus subtilis liquid culture solution to LB seed culture solution, and culture at a constant temperature of 37° C. for 10 to 18 hours with shaking.

[0045] b. Preparation of pure culture of Lactobacillus plantarum seeds: transfer activated Lactobacillus plantarum liquid culture solution to MRS seed culture

[0046] Nutrient solution, culture at 37°C for 10-18 hours.

[0047] B. Crushing of fermentation raw materials

[0048] Take 10-15 parts of corn, 10-15 parts of bran, 15-25 parts of soybean meal, 35-45 parts of palm meal, 10-15 parts of subflour, 1-2 parts of urea, 0.5-1 part of glucose, mix and grind , sieved, the fineness is 20-40 mesh.

[0049] D. Mixing water for fermentation raw materials

[0050] The rat...

Embodiment 2

[0056] Embodiment 2 multi-strain anaerobic fermentation feed

[0057] The specific implementation steps are as follows:

[0058] A. Preparation of Seed Solution

[0059] a. Preparation of pure culture of Bacillus subtilis seeds: transfer 1ml to 2ml of activated Bacillus subtilis liquid culture solution to 50ml LB seed culture solution, and culture at a constant temperature of 37°C for 10 to 18 hours.

[0060] b. Preparation of pure culture of Lactobacillus plantarum seeds: transfer 1ml-2ml of activated Lactobacillus plantarum liquid culture solution to 50ml MRS seed culture solution, and culture at 37°C for 10-18 hours.

[0061] c. Preparation of pure culture of brewer's yeast seeds: transfer 1ml-2ml of activated brewer's yeast liquid culture solution to 50ml of YPD seed culture solution, and culture at 28-30° C. for 12-18 hours.

[0062] B. Crushing of fermentation raw materials

[0063] Take 10-15 parts of corn, 10-15 parts of bran, 15-25 parts of soybean meal, 35-45 part...

Embodiment 3

[0070] Embodiment 3 The method for making feed of the present invention

[0071] The specific implementation steps are as follows:

[0072] A. Activation of strains

[0073] a. Activation of Rhizopus: Pick the spores of Rhizopus from the slant of the test tube stored in the refrigerator at 0-4°C, inoculate them on the potato glucose slant test tube medium, cultivate them at a constant temperature of 28-30°C for 24-48 hours, and pick new bacteria The filaments were transferred to the potato glucose slant test tube culture medium, and cultured at a constant temperature of 28-30°C for 24-48h.

[0074] b. Activation of Aspergillus oryzae: Pick the spores of Aspergillus oryzae from the slant of the test tube stored in the refrigerator at 0-4°C, inoculate it on the potato glucose slant test tube medium, culture at a constant temperature of 28-30°C for 24-48 hours, and pick new bacteria The filaments were transferred to the potato glucose slant test tube culture medium, and culture...

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Abstract

The invention discloses a method of a two-step united multi-strain fermented feed, and belongs to the technical field of feeds. The fermented feed disclosed by the invention is obtained through two steps of aerobic fermentation and anaerobic fermentation by using maize flour, palm dregs, soybean meal, bran and middling as raw materials and by using rhizopus nigricans, aspergillus oryzae, bacillus subtilis, lactobacillus plantarum and beer yeast as fermenting strains. The feed fermented through the method disclosed by the invention has the advantages that the protein content is as high as 30-34% which is increased by 45-62% than that of raw materials, the content of alpha-amylase is as high as 90-110U / g, the enzyme activity of acidic protein is as high as 110-130U / g, and the feed is rich in protein, rich in probiotics and high in enzymic activity. The method disclosed by the invention has the characteristics of high maneuverability, being suitable for large-scale industrial production and the like, and has favorable market prospects.

Description

technical field [0001] The invention relates to a method for multi-strain two-step joint fermentation of feed, belonging to the technical field of feed. Background technique [0002] In recent years, fermented feed has become a research hotspot, and the research on various fermented feeds has been developed to a certain extent. Fermented feed is a biologically fermented feed that uses the action of microorganisms to convert feed materials into microbial cell protein, biologically active small peptide amino acids, microbially active probiotics and compound enzyme preparations. Fermented feed can not only make up for the amino acids that are easily lacking in conventional feed, but also degrade the ingredients of roughage raw materials, improve feed conversion rate, and degrade the antigenic protein in soybean meal. In addition, fermented feed can also reduce the diarrhea rate of pigs and significantly reduce pigs Backfat thickness, lactic acid bacteria fermented feed can als...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 赵建新刘桂香毛丙永闫博文田丰伟范大明赵国忠王刚陈卫张灏
Owner JIANGNAN UNIV
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