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Ready-to-use rapid enzyme immune tissue chemical reagent kit for detecting SCML2

A technology of histochemistry and enzyme immunohistochemistry, applied in the field of medical biology, can solve the problem of no ready-to-use rapid enzyme immunohistochemistry kit, and achieve the effect of improving detection sensitivity and localization, reducing workload and good quality control.

Active Publication Date: 2015-10-21
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant report in the world, and there is no ready-to-use rapid enzyme immunohistochemistry kit specifically for the detection of SCML2. Therefore, the development and application of this kit fills the gap in this regard

Method used

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  • Ready-to-use rapid enzyme immune tissue chemical reagent kit for detecting SCML2

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Embodiment Construction

[0043] 1. 3%H 2 o 2 Deionized water container, 2. Powder type antigen retrieval solution container, 3. HRP enzyme-labeled anti-mouse IgG polymer container, 4. Diaminobenzidine container, 5. Monoclonal mouse anti-human SCML2 antibody container, 6. Polymer Enhancer container, 7, powder type phosphate buffer container, 8, box body

[0044] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0045] Box body 8 is arranged among the present invention, and following container is included in box body 8:

[0046] (1) 3%H2 o 2 Deionized water container 1;

[0047] (2) Container 2 of powdered antigen retrieval solution;

[0048] (3) Powder type phosphate buffer container 7;

[0049] (4) Monoclonal mouse anti-human SCML2 antibody container 5;

[0050] (5) Polymer reinforcing age...

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Abstract

The invention discloses a ready-to-use rapid enzyme immune tissue chemical reagent kit for detecting SCML2. The ready-to-use rapid enzyme immune tissue chemical reagent kit comprises a kit body, the kit body is provided with a 3% H2O2 deionized water container, a powder type antigen repair solution container and a powder type phosphate buffer solution container, and the kit body is further provided with a monoclonal mouse anti-human SCML2 antibody container, a polymer enhancer container, an HRP enzyme-labeled mouse IgG polymer container and a diaminobenzidine container; a reagent in the reagent kit comprises the components of 3% H2O2, powder type antigen repair solutions, powder type phosphate buffer solutions, monoclonal mouse anti-human SCML2 antibodies, polymer enhancers, HRP enzyme-labeled mouse IgG polymers and diaminobenzidine. The ready-to-use rapid enzyme immune tissue chemical reagent kit for detecting SCML2 has the advantages that the detection operation process is greatly simplified, and it can be guaranteed that the detection result has good quality control; the ready-to-use rapid enzyme immune tissue chemical reagent kit for detecting SCML2 further has the advantages of being simple, fast to use, sensitive, universal, good in reproducibility and low in cost.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and in particular relates to a ready-to-use fast method for detecting SCML2 [0002] Fast Enzyme Immunohistochemistry Kit. Background technique [0003] SCML2 was first identified on the Drosophila SCM gene Xp22 chromosome by Montini et al. in 1999, with a total length of about 15Kb and a highly conserved intron and exon structure at the C-terminus. SCML2 is widely expressed in mammals and encodes a 700 amino acid protein. SCML2 is a member of the polycomb gene family. The polycomb family genes encode a group of chromosome binding proteins. Studies have shown that it plays a key role in embryonic development, and it can inhibit the activation of trxG protein on embryonic development. Most of the polycomb family genes in mammals are homologous to Drosophila. When the polycomb family genes in mice are knocked out, the mice have hematopoietic defects, neural crest defects, and heart abnormalit...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/535
CPCG01N33/535G01N33/577G01N33/6803G01N2333/47
Inventor 肖明兵倪润洲江枫黄华陆翠华倪温慨
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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