A kind of differential protein in the serum of dairy cows in early pregnancy stage and its application
A technology of differential protein and early pregnancy, applied in the direction of hemoglobin/myoglobin, immunoglobulin, anti-enzyme immunoglobulin, etc., can solve the problems of inaccurately judging whether a cow is pregnant, inconsistent protein expression trend, difficult to troubleshoot, etc., to achieve Improve pregnancy rate and reproductive efficiency, reduce the risk of embryo loss, and promote the effect of pregnancy maintenance
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[0044] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
[0045] see figure 1 As shown, the embodiment of the present invention provides a differential protein in the serum of the early pregnancy stage of a dairy cow and its use. The method for separating and identifying the serum protein of a fertilized dairy cow includes the following steps:
[0046] S1: Preparation of protein samples
[0047] S101: Perform artificial insemination on several cows, collect blood samples after 0 days, 14 days, 18 days, 21 days, and 28 days after artificial insemination of each cow. After centrifugation for 15 min, the supernatant was aspirated to obtain serum.
[0048] S102: Use the rectal detection method to determine whether the dairy cows that have been artificially inseminated for 60 days are pregnant. According to the results of the rectal examination, randomly select blood samples from 9 pregnant dairy cows 14 ...
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