3D cell printing system and printing method thereof

Abstract

The invention discloses a 3D cell printing system which comprises a main control system, cell printing workbenches and a cell control medium system, wherein the cell printing workbenches include a first workbench and a second workbench, the second workbench is positioned below the first workbench, a plurality of cell array mounting holes are distributed in the first workbench, cell arrays are positioned in the cell array mounting holes, the cell control medium system is used for printing the cell arrays, an extracellular matrix material printing system is installed on the first workbench, a forming workbench is installed in the second workbench, the movement of the cell printing workbenches, the operation of the extracellular matrix material printing system and the operation of the cell control medium system are all controlled by the main control system. Additionally, the invention further discloses a printing method of the system. According to the 3D cell printing system, tissues or organs of human bodies or animals can be constructed in vitro, and individual cell and extracellular matrix distribution can be accurately controlled.

Description

technical field [0001] The invention relates to cell printing technology, in particular to a 3D cell printing system and a printing method thereof. Background technique [0002] Cell printing technology is a new technology that combines rapid prototyping technology and biomanufacturing technology. Scientists have now developed 3D bioprinters that can print skin, cartilage, bones and other organs of the body. The cell printing process is shown in Figure 1. Cell printing technology has attracted extensive attention from scholars from all over the world. One of the challenges facing bioprinters is to print multicellular human or animal organs, since any printed organ or body tissue needs to be connected to the body's blood vessels. The current bioprinting technology mainly focuses on research on new molding methods, cell scaffold materials, improvement of molding accuracy, and mechanical properties of the molding scaffolds. [0003] There are many kinds of cell printing tec...

AI Technical Summary

Problems solved by technology

The current bioprinting technology still has many shortcomings in the construction of large soft tissues and internal organs: 1) The density of planted cells is limited, and the growth of new tissues is slow; 2) Different from the three-dimensional balanced living environment in natural tissues, cells The surface of the stent grows inward, which is not conducive to the mutual contact of cells, the development and functional expression of corresponding organs; 3) Insufficient vascularization, no good vascularization solution, resulting in reduced penetration of nutrients inside the stent, and cells cannot get Sufficient nutrients, so large soft tissues and internal organs that require sufficient blood supply cannot be achieved; 4) Different cells cannot be positioned in different spatial positions, and large soft tissues and internal organs often contain a variety of cells , different cells have unique spatial arrangement characteristics

For example, the patent CN201310294332.0 discloses a cell printing method and a cell printing system. This technical solution uses the same micro-nozzle to realize the absorption and printing of various cells, avoiding the increase in equipment responsibility due to the use of multiple groups of micro-nozzles. It avoids the complexity of the operation process caused by replacing the micro-nozzle, but this technical solution still cannot solve the above-mentioned problems well

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