Method for cultivating microalgae to generate hydrogen

A technology for producing hydrogen and microalgae, applied in biological engineering and fields, can solve the problems of low efficiency and short duration of hydrogen production by microalgae, and achieve the effect of simple preparation process and improved hydrogen production efficiency

Active Publication Date: 2015-10-07
杭州瑞藻科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for cultivating microalgae to produce hydrogen, realizes the sustainable photohydrogen production of microalgae under natural aerobic conditions, and overcomes the problems of short hydrogen production duration and low efficiency of microalgae

Method used

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  • Method for cultivating microalgae to generate hydrogen
  • Method for cultivating microalgae to generate hydrogen
  • Method for cultivating microalgae to generate hydrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The commercialized Chlorella pyrenoidosa was selected as the experimental object.

[0037] Add polydimethyldiallyl ammonium chloride (PDADMAC) in an amount of 1 g / L to the logarithmic growth phase Chlorella cell solution (cell density 1.2 × 10 8 cells / mL) for surface modification to positively charge the surface. Then centrifuge and wash, then centrifuge again, and finally mix the 5mM silicic acid solution with the centrifuged chlorella cells quickly, blow them away, put them in a beaker and stir for 30min. Collect Chlorella cells, wash and resuspend with TAP medium, then aliquot to a volume of 60 cm 3 In sealed tubes, 30mL of algae liquid per tube. Taking the chlorella in natural state as the control, they were divided into two groups. at 100μE·m -2 ·s -1 Exposure to light for 48 hours.

[0038] Experiment 1. The natural state of Chlorella pyrenoidosa and aggregated Chlorella pyrenoidosa were observed under an optical microscope and a scanning electron microscope...

Embodiment 2

[0047] The selected algal species is consistent with that in Example 1, which is Chlorella pyrenoidosa.

[0048] Add polydimethyldiallyl ammonium chloride in the amount of 0.5g / L to the Chlorella cell solution in the logarithmic growth phase (cell density 1.2×10 8 cells / mL) for surface modification to positively charge the surface. Then centrifuge and wash, then centrifuge again, and finally mix the 5mM silicic acid solution with the centrifuged chlorella cells quickly, blow them away, put them in a beaker and stir for 30min. Collect Chlorella cells, wash and resuspend with TAP medium, then aliquot to a volume of 60 cm 3 In sealed tubes, 30mL of algae liquid per tube. at 100μE·m -2 ·s -1 Irradiate under light for 48 hours, and measure the accumulated amount of hydrogen in the headspace of the sealed tube.

Embodiment 3

[0050] The selected algal species is consistent with that in Example 1, which is Chlorella pyrenoidosa.

[0051] Add polydimethyldiallyl ammonium chloride to the Chlorella cell solution in the logarithmic growth phase in an amount of 1.5g / L (cell density 1.2×10 8 cells / mL) for surface modification to positively charge the surface. Then centrifuge and wash, then centrifuge again, and finally mix the 5mM silicic acid solution with the centrifuged chlorella cells quickly, blow them away, put them in a beaker and stir for 30min. Collect Chlorella cells, wash and resuspend with TAP medium, then aliquot to a volume of 60 cm 3 In sealed tubes, 30mL of algae liquid per tube. at 100μE·m -2 ·s -1 Irradiate under light for 48 hours, and measure the accumulated amount of hydrogen in the headspace of the sealed tube.

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Abstract

The invention provides a method for cultivating microalgae to generate hydrogen. The method includes the steps that firstly, an aggregate of the microalgae is constructed, then the aggregate of the microalgae is resuspended in a culture medium, and cultivation is conducted under the illumination condition to generate hydrogen. A method for constructing the aggregate of the microalgae includes the following steps that firstly, microalgae cells are cultivated to a logarithmic phase, and the cell density is not smaller than 1.2*108 cells / mL; secondly, positive ion polyelectrolyte carrying amidogen is added to a microalgae cell solution with the amount of 0.5 g / L-2 g / L to conduct surface modification; thirdly, the microalgae cells are collected and are mixed with a 2-10 mM silicic acid solution, and stirring is conducted until the aggregate of the microalgae is formed. By means of the method, sustainable light hydrogen production of the microalgae under the natural aerobic condition is achieved, and the problems that the hydrogen production sustainable time is short and efficiency is low are solved.

Description

technical field [0001] The invention relates to the field of bioengineering and technology, in particular to a method for cultivating microalgae to produce hydrogen. Background technique [0002] Hydrogen has long been considered an ideal and promising alternative to fossil fuels because it burns water, is environmentally friendly, and has high energy efficiency. At present, the industrial production of hydrogen mainly comes from the steam cracking of hydrocarbons and the electrolysis of water, but the production methods of these hydrogen are energy-costly and unsustainable. The green production of hydrogen has always been a challenge. Photobiological hydrogen production is considered to be an ideal sustainable hydrogen production pathway. In nature, photosynthetic microorganisms, especially green algae, can use photosynthetic system and hydrogenase to photolyze water into hydrogen and oxygen (A.Hemschemeier, T.Happe.Alternative photosynthetic electron transport pathways d...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N1/12C12R1/89
Inventor 熊威赵小红马为民徐旭荣唐睿康
Owner 杭州瑞藻科技有限公司
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