Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof

A herbicide, 4-D technology, applied in the field of microorganisms, can solve the problems of crop damage economy, impact on food safety, strong adsorption and other problems, so as to solve the phytotoxicity of next crops and relieve the phytotoxicity of subsequent crops , the effect of strong resistance

Active Publication Date: 2015-10-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2,4‐D degrades slowly and has strong adsorption, so it is easy to remain
In addition, the phytotoxicity of next crops caused by 2,4‐D residues also caused serious economic losses and affected food security.

Method used

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  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof
  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof
  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The invention provides a bacterial strain capable of efficiently degrading (mineralizing) 2,4-D and the bacterial agent produced therefrom. The bacterial strain used is Gram-negative bacteria LZ35, which is isolated from the soil of the Lvlilai Pesticide Factory in Kunshan, Jiangsu. . The specific isolation and screening method of the strain is as follows: take 5.0g of soil sample and add it to 100ml of inorganic salt liquid medium with 0.1% yeast powder added, add 2,4‐D to a final concentration of 50ppm, and carry out enrichment culture at 30°C. Shaker speed is 150rpm·min ‐1 , inoculated into fresh inorganic salt liquid medium with 10% inoculation amount every 7 days, and subcultured continuously for 3 times. Then inoculate 1% of the inoculum into fresh 20ml inorganic salt liquid medium with 2,4‐D final concentration of 50ppm, and culture for 5 days. The content of 2,4‐D was detected simultaneously by UV scanner and liquid chromatography, and it was found that 2,4‐D ...

Embodiment 2

[0040] Activate the original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seed is inoculated in a 1000ml shaker flask containing 200ml of broth medium (3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 ml of water, pH 7.0-7.2), constant temperature shaking culture to logarithmic phase, ready to inoculate seeds Can. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate to the logarithmic growth phase, an...

Embodiment 3

[0042] Activate the original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 500ml broth medium (3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), and cultured with constant temperature shaking until the logarithmic phase. The bacteria were collected by centrifugation, and the bacteria were resuspended in sterile water. Then take the fresh test soil, add 2,4‐D aqueous solution, mix well, so that the concentration of 2,4‐D in the soil is 50 mg kg ‐1 and 100mg·kg ‐1 dry soil. At the same time, the treatment group was inoculated with fresh LZ35 strain suspension in the soil, and the blank control group was added with sterile water, which was also stirred evenly. The mixed soil is divided into disposable plas...

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Abstract

The invention discloses a degradation strain of herbicide 2, 4-D (2, 4-dichlorophenoxy acetic acid), a produced inoculum and application thereof. The strain is preserved in China Center For Type Culture Collection on April 15, 2015, and the strain preservation number is CCTCC NO:M2015235. The strain is gram staining reaction negative strain LZ35, which is identified as Achromobacter sp. The 2, 4-D degradation strain LZ35 can be applied to degradation of the herbicide 2, 4-D. A degradation inoculum produced from the 2, 4-D degradation strain LZ35 can degrade 99% of 100mg / L 2, 4-D in a liquid culture medium within 2 days, and direct application of the inoculum can significantly reduce crop phytotoxicity, solve the exceeding herbicide residue problem in agricultural production and produce non-toxic and nuisanceless green agricultural products.

Description

technical field [0001] The invention belongs to the field of microbes, and provides a herbicide 2,4-D degrading bacterial strain as well as the bacterial agent for its production and application. technical background [0002] Organochlorine pesticides are one of the most important and effective pesticides used before the 1980s. Because of their low price, high efficiency and broad spectrum, they have been widely used in the world. Among them, phenoxyacetic acid herbicides are produced and used on a large scale because of their low cost and high speed. 2,4‐D (2,4‐dichlorophenoxyacetic acid; 2,4‐Dichlorophenyloxy acetic acid) is a representative substance for the synthesis of auxin, and it is also a commonly used herbicide, which plays an important role in agriculture and forestry. It plays a significant role, especially widely used in the preservation of fruits (such as citrus). 2,4‐D can be used as a plant growth regulator at low concentrations below 30ppm, and as a herbic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/025A62D101/04
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C12N1/205C12R2001/025
Inventor 蒋建东张龙杜方岭周庆新王军华陈凯李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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