Carya illinoensis cell embryo tissue culture method

Abstract

The invention discloses a carya illinoensis cell embryogenesis method. The method comprises steps of material selection, sterilization, induction culture of test-tube bud seedlings, induction of somatic embryo, multiplication culture, maturation culture and sprouting of the somatic embryo; the culture is carried out in a somatic embryo induction, multiplication, maturation and sprouting culture medium. The method has high multiplication coefficient, the multiplication time is not subjected to the limitation of seasons, the harm of used materials on a parent body is small, the trouble of diseases and injurious pests can be avoided, excellent characters of original plants can be maintained, and an important application value in production and scientific research of carya illinoensis seedlings can be realized.

Description

technical field [0001] The invention relates to a tissue culture method of pecan, in particular to a somatic embryo tissue culture method of pecan. Background technique [0002] Thin-shelled hickory, also known as American hickory, is a species of Juglandaceae and Hickory. Native to the United States and Mexico, it is one of the most important oil-bearing dried fruit tree species in the world. Its shell is thin and easy to peel, its kernels are thick and fleshy, and it has a delicious taste and high nutritional value. The trunk is straight, the tree shape is beautiful, the roots are deep and the leaves are luxuriant, and it is an excellent tree species for greening and gardening around. At the same time, the wood is tough and dense, with dark red heartwood, yellow-white sapwood, high elasticity, and is not easy to deform and crack. It is an excellent timber tree species for construction and high-end furniture. Therefore, pecan is an excellent economic tree species with wid...

AI Technical Summary

Problems solved by technology

However, whether it is spring sowing or autumn sowing, it is difficult for annual seedlings to reach the level of grafting, and the growth is slow.

In order to make the thin-shelled pecans embark on the process of improving varieties and building bases as soon as possible, while accelerating germination and cultivating improved varieties, we started to tackle key problems in rootstock grafting technology. However, due to the low survival rate of grafting, the supply of grafted seedlings was in short supply. However, the seedlings have defects such as late production, small nuts, and poor adaptability, and are not popular in the market. Therefore, there are two key factors restricting the development of production. One is the lack of sufficient seedlings of good varieties, and the other is the lack of scientific systems. cultivation techniques

[0004] Those skilled in the art are also engaged in the work of planting a large amount of Carya carya seedlings, and have focused on asexual propagation experiments such as Carya carya cuttings and grafting, but the reproduction coefficient is low, and the quality of seedlings is uneven. In the prior art, Carya carya The asexual reproduction technology still stays in the stage of tentative experiments, and has not yet formed a mature method that can be widely promoted and repeatedly verified.

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