Bacillus amyloliquefaciens HN-11 and microbial inoculums thereof

Abstract

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

Description

technical field [0001] The invention belongs to the fields of biological pesticides and plant protection, and in particular relates to a preparation method of bacillus amyloliquefaciens strain HN-11 and its microbial agent, and its application in the control of plant diseases and insect pests. Background technique [0002] Neem residue is the by-product of the extraction of azadirachtin from the neem seeds of the insecticidal plant. Due to the low utilization rate, it is usually discarded as waste, which greatly wastes the raw materials that can be used as plant pest control products. Our research finds that neem slag itself still contains a small amount of active ingredients with insecticidal and antibacterial effects, but in the prior art, if the neem slag is used directly, the disease prevention effect is not good in a small amount, and a large amount (greater than 5%) will be used. It will cause plant damage to seedlings, so it is necessary to complete the decomposing pr...

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Problems solved by technology

[0005] The object of the invention is to provide a kind of Bacillus amyloliquefaciens HN-11 compatible with neem dregs and the preparation method of novel microbial agent thereof according to the above-mentioned deficiencies in the prior art. The effect is not good, and a large number of seedlings are injured, so the organic combination of bio-control bacteria and neem residue expands the spectrum of control

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