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Preparation method of molecular imprinting sorptive extraction stirring bar by using alternative template

A technology of molecular imprinting and replacing templates, which is applied in the fields of analytical chemistry and sample pretreatment, can solve the problems of template molecule leakage, affecting the accuracy of target analytes, etc., and achieve the effect of avoiding wear and tear

Inactive Publication Date: 2015-09-23
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The technical problem to be solved in the present invention is: traditional molecularly imprinted polymers have the ability to selectively recognize template molecules, but in the process of extraction and desorption of trace substances, the leakage of template molecules is prone to occur, which affects the accuracy of target analytes ; The object of the present invention is to provide a method for preparing a molecularly imprinted adsorption extraction stirring rod that replaces the template

Method used

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  • Preparation method of molecular imprinting sorptive extraction stirring bar by using alternative template
  • Preparation method of molecular imprinting sorptive extraction stirring bar by using alternative template
  • Preparation method of molecular imprinting sorptive extraction stirring bar by using alternative template

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Embodiment 1

[0035] The preparation method of the molecularly imprinted adsorption and extraction stirring rod for replacing the template, the specific steps are as follows:

[0036] Step 1, capping one end of a glass capillary with an inner diameter of 1.4mm, and preparing one end of a glass capillary with an outer diameter of 1.0mm;

[0037] Step 2. Dissolve 150mg of p-tert-butylphenol (1.0mmol) as a template molecule, template molecule and 284mg of functional monomer (4mmol) in 5mL of polymerization solvent, ultrasonically mix evenly and let it stand for 15h to obtain a mixed solution, add to the mixed solution Add 3.8mL crosslinking agent (20mmol) and 37.6mg initiator (0.23mmol) in turn and mix well to obtain an assembly solution, in which the molar ratio of template molecule, functional monomer and crosslinking agent is 1:4:20, and the template in the mixed solution The concentration of the molecule is 0.114mmol / mL, and then ultrasonic deoxygenation; wherein the functional monomer is ...

Embodiment 2

[0046] The preparation method of the molecularly imprinted adsorption and extraction stirring rod for replacing the template, the specific steps are as follows:

[0047] Step 1, capping one end of a glass capillary with an inner diameter of 1.4mm, and preparing one end of a glass capillary with an outer diameter of 1.0mm;

[0048] Step 2. Dissolve 150mg of p-tert-butylphenol (1.0mmol) as a template molecule, template molecule and functional monomer (3.5mmol) in 7.5mL of polymerization solvent, ultrasonically mix evenly and let stand for 10h to obtain a mixed solution. Add cross-linking agent (13mmol) and initiator (0.20mmol) in sequence and mix well to obtain an assembly solution, wherein the molar ratio of template molecules, functional monomers, and cross-linking agents is 1:3.5:13, and the concentration of template molecules in the mixed solution 0.134mmol / mL, and then ultrasonically deoxygenated; wherein the functional monomer is acrylamide; the crosslinking agent is ethyl...

Embodiment 3

[0052] The preparation method of the molecularly imprinted adsorption and extraction stirring rod for replacing the template, the specific steps are as follows:

[0053] Step 1, capping one end of a glass capillary with an inner diameter of 1.4mm, and preparing one end of a glass capillary with an outer diameter of 1.0mm;

[0054] Step 2. Dissolve 150mg of p-tert-butylphenol (1.0mmol) as a template molecule, template molecule and functional monomer (4mmol) in 13.7mL of polymerization solvent, ultrasonically mix evenly and let stand for 10h to obtain a mixed solution. Add crosslinking agent (46mmol) and initiator (0.36mmol) in sequence and mix well to obtain an assembly solution, in which the molar ratio of template molecules, functional monomers, and crosslinking agents is 1:4:46, and the concentration of template molecules in the mixed solution is 0.073mmol / mL, and then ultrasonically deoxygenated; wherein the functional monomer is acrylamide; the crosslinking agent is ethyle...

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Abstract

The invention relates to a preparation method of a molecular imprinting sorptive extraction stirring bar by using an alternative template, belonging to the technical field of analytical chemistry and sample pretreatment. The method includes firstly using p-tert-butylphenol as a template molecule, dissolving the template molecule and a functional monomer in a solvent, uniformly mixing by ultrasound and standing, then adding a crosslinking agent and an initiator to obtain an assembly solution, and removing oxygen by ultrasound; injecting the assembly solution into a glass capillary with an inner diameter of 1.4mm, then inserting into a glass capillary with an outer diameter of 1.0mm, performing water bath heating for polymerization, removing the larger glass capillary, taking out the small glass capillary, cutting into appropriate length with a blade, inserting a iron core and sealing ends, sleeving silicon tubes on both ends, and cleaning with methanol, to obtain the molecular imprinting sorptive extraction stirring bar using p-tert-butylphenol as the alternative template. The obtained stirring bar can be applied to extraction analysis of trace amount of environmental estrogen bisphenol A in a water sample.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted adsorption extraction stirring rod replacing a template, and belongs to the technical field of analytical chemistry and sample pretreatment. Background technique [0002] Compared with the rapid development of instrumental analysis, the link of sample pretreatment has been neglected to a certain extent, and has become a bottleneck problem restricting the development of analytical chemistry. [1] . In the past twenty or thirty years, various novel sample pretreatment methods and techniques have been proposed, such as solid-phase microextraction [2] , Stir bar adsorption extraction [3] Wait. However, these novel sample pretreatment methods do not have specific selectivity during the sample enrichment process, and both impurities and matrix may be enriched at the same time. [0003] Molecular imprinting technology has the characteristics of predetermined configuration, specific se...

Claims

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Application Information

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IPC IPC(8): B01J20/285B01J20/30G01N1/34G01N1/40
Inventor 许志刚冯锋刘瑞梅陈果林刘智敏
Owner KUNMING UNIV OF SCI & TECH
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