Flow cytometry detection fluid circuit system and flow cytometry detection method

Abstract

The invention relates to the technical field of human blood detection. A flow cytometry detection fluid circuit system comprises a diluent injection system, a hemolysin and blood sample injection system and a pressure system, wherein for the diluent injection system, a diluent injector is driven by a motor, and the diluent injection system is connected with a diluent barrel to suck a diluent to inject to a flowing chamber, a reaction tank and a sampling needle cleaning device; for the hemolysin and blood sample injection system, a hemolytic agent injector is driven by a motor, the hemolysin and blood sample injection system is used for sucking the hemolytic agent to inject to the reaction tank, and the sample injection sucking collects a distribution blood sample to inject to the reaction tank; for the pressure system, a diaphragm pump is connected with a pressure chamber to form positive and negative pressures, the pressure system is used for pumping waste liquid of the sampling needle cleaning device, sucking a sample in the reaction tank to a sample injection port of the flowing chamber, carrying out gas blowing stirring and evacuation on the reaction tank, and pumping the diluent to the reaction tank. According to the invention, the number of reaction tanks is reduced, system structure and complexity are simplified, and miniaturization and low cost are realized for machines.

Description

technical field [0001] The invention relates to the technical field of human blood detection, in particular to a flow cytometry detection fluid circuit system and a flow cytometry detection method. Background technique [0002] The fluid circuit system of five-category hematology analyzers currently on the market is mainly composed of impedance channel modules and DIFF channel modules. The modules include DIFF incubation reaction pool and flow chamber components and their subsidiary piping systems, as well as blood collection and separation modules, waste liquid discharge and cleaning modules, and positive and negative pressure building modules. These modules are relatively independent, and require corresponding syringes and their motors, valves, pumps, or air sources and pressure stabilizing devices. Meet the needs of grassroots users. Patent application number CN201410173070.7) is that the functions of the sheath flow syringe and the diluent syringe are combined into a d...

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Problems solved by technology

These modules are relatively independent, and require corresponding syringes and their motors, valves, pumps, or air sources and pressure stabilizing devices. Meet the needs of grassroots users

Patent application number CN201410173070.7) is that the functions of the sheath flow syringe and the diluent syringe are combined into a diluent syringe, the functions of the sample flow syringe and the sampling syringe are combined into a sample syringe, the positive pressure building system and the waste liquid discharge system are combined, and the diluent Syringes, sample syringes, and hemolytic agent syringes share one motor, and the positive pressure building system and waste liquid discharge system are driven by the same motor. All syringes are driven by the same motor, which affects the flexibility of timing control and affects the testing speed of the whole machine. Syringes There is no pressure sensing unit in the main circuit. If there is a blockage in the pipeline, it will easily lead to high-pressure tube collapse. The impedance counting of the counting tank has no rear tank cleaning part, which will easily affect the next measurement result. The outlet of the flow chamber is directly connected to the negative pressure tank. If Simultaneous implementation of impedance counting and sheath flow will easily affect the stability of impedance counting negative pressure, resulting in inaccurate counting results, which can only be performed in series, affecting speed measurement

Sampling needle outer wall cleaning waste liquid valve and negative pressure chamber emptying share a three-way solenoid valve. This valve cannot be switched when the negative pressure chamber is maintaining pressure and counting. Washing the outer wall of the needle will cause pressure fluctuations, and the channel of the outer wall of the cleaning needle and the discharge of the reaction pool will There is no filtering device in the empty pipeline, which increases the probability of valve blockage of the common valve

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