Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation

A technology of bagasse and succinic acid is applied in the field of bioengineering, which can solve the problems of increased cost and low yield, and achieve the effects of increasing concentration, protecting the environment, and requiring low fermentation equipment.

Inactive Publication Date: 2015-09-09
SHANDONG XINGQIANG CHEM IND TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of succinic acid fermented by these methods is low, which increases the cost of subsequent extraction

Method used

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  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation
  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation
  • Method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: dilute alkali pretreatment bagasse

[0035] Take 20 g of crushed and dried bagasse, mix it with 300 mL of NaOH solution with a mass concentration of 1%, pretreat the bagasse at a high temperature of 121°C for 2 hours, filter and wash the bagasse until neutral, and dry at 65°C to obtain 13g of pretreated bagasse. The processed bagasse has a weight loss of 35%, a total cellulose content of 87.6%, and its composition is shown in Table 1.

[0036] Table 1 Components of alkaline pretreated bagasse measured by NREL method

[0037]

Embodiment 2

[0038] Example 2: Multi-enzyme synergistic hydrolysis of bagasse after alkali treatment

[0039] Weigh 3g of the bagasse that has been pretreated with alkali, transfer it to a 150mL conical flask, add cellulase 25FPU.g -1 , Xylanase 85U.g -1 , β-glucanase 40U.g -1 , Pectinase 25U.g -1 , add pH 5.0 citric acid buffer solution, set the volume to 40mL, perform hydrolysis on a water bath shaker at 50°C for 40h, and take a sample every 4h. Multi-enzyme synergistic hydrolysis time figure 2 . After 40 hours of hydrolysis, the reducing sugar concentration in the hydrolyzate is 65.6g.L -1 , the total cellulose hydrolysis efficiency of bagasse reaches up to 90%, and the concentration of glucose in bagasse hydrolyzate is 45.06g.L -1 , the concentration of xylose is 14.92g.L -1 , the concentration of glucuronic acid is 5.62g.L -1 , the concentration ratio of glucose and xylose is close to 3:1. The multi-enzyme synergistic hydrolysis pretreatment time curve of bagasse is as follo...

Embodiment 3

[0040] Embodiment 3: sugarcane bagasse hydrolyzate fed-batch fermentation produces succinic acid

[0041] Strain: Actinobacillus ssuccinogenes F3-21. Medium: bagasse hydrolyzate sugar concentration 55g.L -1 , corn steep liquor concentration 25g.L -1 , anhydrous calcium chloride concentration 0.2g.L -1 , dipotassium hydrogen phosphate concentration 2g.L -1 , sodium dihydrogen phosphate concentration 9.6g.L -1 , magnesium chloride hexahydrate concentration 0.2g.L -1 , sodium sulfide concentration 0.1g.L -1 , adjust the pH to 6.0, and sterilize at 121°C for 20 minutes.

[0042] In a 3L stirred fermenter, fed-batch fermentation is adopted, and the concentration of the initial bagasse hydrolyzed sugar solution is 55g.L -1 , when the residual sugar concentration in the fermentation broth drops to 20g.L -1 When using a peristaltic pump, add the total reducing sugar concentration to 200g.L -1 The concentrated sugar cane bagasse is hydrolyzed to control the sugar concentration...

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Abstract

The invention discloses a method for producing succinic acid by taking sugarcane bagasse as raw materials through fermentation and belongs to the technical field of bioengineering. The method is characterized by comprising the following steps of taking the sugarcane bagasse which is main byproducts in the sugar pressing industry as raw materials, and performing crushing, pretreatment with dilute alkali as well as common hydrolysis on cellulase, xylanase, beta-glucanase and pectinase to obtain a hydrolyzed sugar solution; fermenting the hydrolyzed sugar solution by using actinobacillus succinogenes to produce the succinic acid; meanwhile, residues left by hydrolyzing the sugarcane bagasse are added into fermented solution, so that the fermentation can be repeatedly operated, seed culture can be omitted and furfural in residual liquid obtained by extraction of organic solvent and pretreatment with the dilute alkali can be utilized. According to the method disclosed by the invention, reasonable utilization of hydrolyzed residues of the sugarcane bagasse is realized, and the conventional fermented seed culture link is reduced, so that the fermentation cost is reduced, and the fermentation efficiency is improved; besides, the requirement on fermentation equipment is low, extra improvement on an existing fermentation tank is not needed, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for producing succinic acid by fermenting bagasse as a raw material, and belongs to the technical field of bioengineering. Background technique [0002] Bagasse is an important renewable raw material of lignocellulosic fiber. my country is the country with the third largest planting volume of sugarcane in the world. The south is the main area where sugarcane is planted in China. Tons of bagasse. [0003] The main components in bagasse are cellulose, hemicellulose and lignin. At present, the main uses of bagasse are burning power generation (Light Industry Science and Technology, 2013, (11): 11-12.), as livestock feed (Chinese Patent Application No. 94113455.5), replacing traditional wood resources as papermaking and producing high-density materials ( China Plastics, 2008, (02): 57-61), as a medium for edible fungi (Edible Fungi, 1980, (02): 12-16), and the development and production of high value-added products: such ...

Claims

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Application Information

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IPC IPC(8): C12P7/46C07D307/50C12R1/01
CPCC12P7/46
Inventor 郑璞淘声涛查鑫华
Owner SHANDONG XINGQIANG CHEM IND TECH RES INST CO LTD
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