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Haematococcus pluvialis ENN71 and cultural method and application of haematococcus pluvialis ENN71

A technology of Haematococcus pluvialis and wild strains, applied in the field of microorganisms, can solve the problems of difficulty in meeting the demand for astaxanthin, shorten the accumulation cycle of astaxanthin, and the long cycle of astaxanthin, and achieve stable performance, short growth cycle, The effect of strong environmental adaptability

Active Publication Date: 2015-09-09
ENN SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the production of astaxanthin, due to the slow growth rate of Haematococcus pluvialis and the long cycle of accumulating astaxanthin, it is difficult to meet the market demand for astaxanthin. Algal strains shorten the accumulation cycle of astaxanthin, which is of great significance to the increase of natural astaxanthin production

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  • Haematococcus pluvialis ENN71 and cultural method and application of haematococcus pluvialis ENN71
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  • Haematococcus pluvialis ENN71 and cultural method and application of haematococcus pluvialis ENN71

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specific Embodiment approach

[0046] These examples are merely examples for specifically illustrating the present invention, and those skilled in the art will understand that the scope of the present invention is not limited by these examples, comparative examples, and test examples.

[0047] In an embodiment provided by the present invention, the screening and obtaining of the mutant strain of Haematococcus pluvialis ENN71 is specifically as follows:

[0048] The algae liquid of wild Haematococcus pluvialis was subjected to ultraviolet mutagenesis twice, EMS chemical mutagenesis treatment, and herbicide was added for repeated pressure treatment, primary screening and secondary screening, and a mutant strain ENN71 with excellent performance and high yield of astaxanthin was obtained. The Haematococcus pluvialis mutant strain ENN71 is preserved in the General Microorganism Center (CGMCC) of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.5147.

[0049] I...

Embodiment 1

[0051] In a column reactor with an inner diameter of 40 mm and a length of 600 mm, cells of ENN71 algae were inoculated in BG11 medium at a cell density of 0.5 g / L, and wild ENN wt was used as a control. During the cultivation process, the light intensity was 100 μmol / m 2 s, the culture temperature is 25-28°C, during the culture period, feed a mixed gas of 1.5-2% carbon dioxide and air into the culture medium, so that the pH value of the culture medium is 7-9, every day Microscopic examination was carried out, and the changes in cell morphology during the whole culture process were similar to those of wild strains. Samples were taken regularly to measure the dry weight of algal cells, and a comparison chart of growth speed curves of algal strains was obtained.

[0052] see figure 1 The comparison chart of the growth rate curve of a strain of Haematococcus pluvialis mutant strain ENN71 and the wild strain ENN wt provided for the embodiment of the present invention, it can be s...

Embodiment 2

[0054] The embodiment 2 is basically the same as the embodiment 1, the only difference is that the ENN71 algae cells are inoculated in the BG11 medium, the cell density is 1.5g / L, and the light intensity during the cultivation is 300 μmol / m 2 · s, the culture temperature is 25-30° C., a mixed gas of 5-10% carbon dioxide and air is introduced into the culture medium, and the pH value of the culture medium is 6.5-8. On the 12th day of cultivation, the Haematococcus pluvialls mutant strain ENN71 and the wild strain ENN wt were sampled to determine the dry weight of the algae cells, and the cultured first day of the Haematococcus pluvialls mutant strain ENN71 and the wild strain ENN wt was obtained. 12 day comparison chart.

[0055] see figure 2 , as can be seen from the figure, compared with the dry weight of algae powder obtained by cultivating ENN71 under conventional conditions for 12 days and the wild strain ENN wt, the growth of the mutant strain ENN71 was significantly be...

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Abstract

The invention relates to the field of microorganisms, and in particular to haematococcus pluvialis ENN71 and a cultural method and application of the haematococcus pluvialis ENN71. The haematococcus pluvialis ENN71 is short in growth period, the content of natural astaxanthin is high, and the inheritance performance is stable. The haematococcus pluvialis ENN71 provided in the embodiment is Haematococcus pluvialis and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation serial number is CGMCC No.5147, and the preservation date is August 17, 2011.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Haematococcus pluvialis ENN71 and its cultivation method and application. Background technique [0002] Astaxanthin is a kind of carotenoid, which is the strongest antioxidant in the world. It can effectively remove free radicals in cells, reduce the accumulation of aging cells, and has the functions of anti-aging, relieving exercise fatigue, enhancing immunity, and inhibiting tumors. effect. Astaxanthin is divided into natural astaxanthin and synthetic astaxanthin. However, the stability, biological safety and absorption effect of natural astaxanthin are better than artificial astaxanthin. The content of astaxanthin can reach 1.5-3%, and it is regarded as a "concentrated product" of natural astaxanthin. In view of the remarkable physiological functions of astaxanthin, it has been widely used in medical care, beauty and skin care and other industries. [0003] As a nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
Inventor 吴洪陈传红尹顺吉朱振旗李青
Owner ENN SCI & TECH DEV
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