Ointment applied to pruning wounds of diseased branches of blueberry plants
A technology of ointment and diseased branches, which can be used in animal repellents, botanical equipment and methods, biocides, etc., and can solve problems that have not been reported before.
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Embodiment 1
[0018] Embodiment 1: A kind of blueberry diseased branch cuts and smears the ointment, taking the production of 100L product as example:
[0019] 1) Measure 37.5L of Vaseline and 37.2L of lanolin and melt in a water bath at 40°C;
[0020] 2) Measure 2L of pyraclostrobin suspension concentrate with a mass fraction of 30%, 10L of chitosan aqueous solution with a mass fraction of 0.5%, and 13L of liquid paraffin, and add them to the molten liquid of vaseline and lanolin and stir for 30 minutes;
[0021] 3) Add 0.3 L portion of fatty alcohol polyoxyethylene ether, and continue stirring for 10 minutes;
[0022] 4) Adjust the pH value of the solution to 6-6.5, keep the viscous colloid, and fill it into a bottle.
Embodiment 2
[0023] Embodiment 2: pyraclostrobin toxicity test
[0024] In order to understand the exact toxicity of different fungicides to blueberry branch blight, the present invention screens effective agents for preventing and controlling blueberry branch blight.
[0025] Three kinds of pathogenic bacteria causing blueberry branch blight, Botryosphaeria dothidea, Phomopsis vaccinii and Pestalotiopsis clavispora (isolated from dead branches in blueberry orchards in Qingdao, Shandong), were collected from natural For the diseased plants, the diseased branches were isolated by the conventional tissue separation method. After the isolated strains were purified and cultured, their pathogenicity was verified by Koch's law, and then the single cells were purified and stored on a PDA slope, and stored in a refrigerator at 4°C for later use.
[0026] Test agents: Ⅰ (250g / L pyraclostrobin should be pyraclostrobin emulsifiable concentrate), Ⅱ (24% carbazole suspension), Ⅲ (25g / L fludioxonil susp...
Embodiment 3
[0034] Example 3: Field efficacy test of different fungicides on blueberry grape stalk blight
[0035] Test agents: Ⅰ (250g / L azoxystrobin suspension 1500 times liquid + 0.5% chitosan water 300 times liquid); Ⅱ (25g / L fludioxonil suspension seed coating 3000 times liquid + 0.5% chitin Polysaccharide water 300 times liquid); Ⅲ (250g / L pyraclostrobin EC 1800 times liquid + 0.5% chitosan water 300 times liquid); Chitosan water solution 300 times) and CK (water control).
[0036] Method: The experiment was carried out in the blueberry plots with disease in previous years in Qingdao blueberry base. The experiment was repeated 3 times (plots), and the plots were randomly arranged. There were 3 trees in each plot, a total of 15 plots, and a total of 45 trees. Immediately after the fruit picking, the first spraying was carried out, and the second spraying was carried out at an interval of 10 days.
[0037] Before spraying, each tree had 40 branches in 4 directions from east to west,...
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