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Method for detecting zolpidem and 6-zolpidem carboxylate serving as zolpidem poisoning markers in urine by using liquid chromatography-tandem mass spectrometry

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no sensitivity and low detection limit, and achieve the effect of strong selectivity, low detection limit and simple operation

Active Publication Date: 2015-09-02
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no detection method for zolpidem and zolpidem 6-carboxylate in the prior art, especially no detection method with high sensitivity and low detection limit

Method used

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  • Method for detecting zolpidem and 6-zolpidem carboxylate serving as zolpidem poisoning markers in urine by using liquid chromatography-tandem mass spectrometry
  • Method for detecting zolpidem and 6-zolpidem carboxylate serving as zolpidem poisoning markers in urine by using liquid chromatography-tandem mass spectrometry
  • Method for detecting zolpidem and 6-zolpidem carboxylate serving as zolpidem poisoning markers in urine by using liquid chromatography-tandem mass spectrometry

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Embodiment 1

[0038] In this embodiment, the method for detecting zolpidem and zolpidem 6-carboxylate, a zolpidem poisoning marker in urine, using liquid chromatography-tandem mass spectrometry, includes the following steps:

[0039] 1. Experimental reagents, instruments and experimental equipment

[0040] 1 experimental reagent

[0041] 1.1 Standards and reagents

[0042] Zolpidem and Zolpidem 6-carboxylate standard substance, content 99wt%, purchased from Canadian Toronto Chemical Company;

[0043] Formic acid, acetonitrile, ammonium acetate, methanol, etc. are chromatographically pure reagents, purchased from Sinopharm;

[0044] Ultrapure water was prepared by Millipore simplicity pure water system.

[0045] 1.2 Preparation of standard solutions and related solvents

[0046] Preparation of standard stock solution: Accurately weigh 10 mg of zolpidem and zolpidem 6-carboxylate standard, dissolve them in methanol, and adjust the volume to the mark to prepare a 1 mg / mL standard stock sol...

Embodiment 2

[0069] The difference between Example 2 and Example 1 is that the chromatographic column is: ACQUITY UPLC HELIC column, 2.1 mm×100 mm, 1.7 μm.

Embodiment 3

[0071] The difference between embodiment 3 and embodiment 1 is that the mobile phase of liquid chromatography is mobile phase combination two: it is composed of phase A and phase B, phase A is 0.05% by volume of acetonitrile, 0.1% by volume of formic acid and The volume fraction of water is 99.85% of the mixed solution 2A, and the phase B is the mixed solution 2B of 0.05% of water, 0.1% of formic acid and 99.85% of acetonitrile.

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Abstract

The invention discloses a method for detecting zolpidem and 6-zolpidem carboxylate serving as zolpidem poisoning markers in urine by using a liquid chromatography-tandem mass spectrometry. The method comprises the following steps of (1) treating a urine sample to obtain urine to be detected; and (2) detecting zolpidem and 6-zolpidem carboxylate in the urine to be detected in the step (1) by using a liquid chromatography-tandem mass spectrometry instrument. The method disclosed by the invention has the advantages of high sensitivity and low detection limit, is simple in operation, high in recovery rate and strong in selectivity, can be used for inspecting and appraising the poisoning of zolpidem in the urine, so that the detection for zolpidem poisoning related cases in inspection of poisoning cases and criminal cases is improved.

Description

technical field [0001] The invention relates to a poison detection method in the field of criminal investigation, in particular to a method for detecting zolpidem poisoning markers zolpidem and 6-carboxylate zolpidem in urine using liquid chromatography-tandem mass spectrometry for the purpose of criminal investigation. Background technique [0002] Zolpidem is one of the representative drugs of the third-generation hypnotics-non-benzodiazepine hypnotics. The trade name is Sinuosi, which is an imidazopyridine hypnotics. As the clinical use of zolpidem has increased, related cases have also emerged in the field of forensic science. Because of its fast absorption, fast metabolism, and short test time, the original body is not easy to detect. Therefore, studying zolpidem and its metabolites and clarifying the laws of biotransformation are of great significance for case testing and forensic science. [0003] The chemical name of zolpidem is N,N,6-trimethyl-2-(4-tolyl)imidazo[1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张蕾萍姚伊人于忠山王芳琳董颖王瑞花常靖栾玉静杜鸿雁崔冠峰候小平
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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