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Method for preparing probitics through two-step fermentation method by using degreased rice bran as raw material

A technology of defatted rice bran and prebiotics, applied in animal feed, animal feed, application, etc., can solve the problem of high product cost, achieve low cost, reduce diarrhea, and facilitate the growth of microorganisms

Inactive Publication Date: 2015-09-02
JIANGSU BAOBAO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the production of prebiotics requires relatively expensive nitrogen sources, such as yeast extract for liquid fermentation, soybean meal for solid fermentation, etc., and the product cost is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add 100kg of defatted rice bran, 54kg of water, 500g of molasses, 500g of dry yeast powder, 100g of dry powder of lactic acid bacteria, and 20g of dry powder of Bacillus subtilis into the fermentation tank, mix well, and control the temperature at 28°C. After entering the tank, the stacking height is 70cm, and the fermentation is about 36 hours at a temperature of 50°C. After the first turning, the thickness of the stacking is reduced by 50cm. After that, turning once a day, the fermentation is completed in 48 hours. Add 20g of cellulase and 20g of protease for enzymolysis, and complete the enzymolysis in 24 hours. After drying at 50°C, it is crushed to make a finished product.

Embodiment 2

[0023] Add 100kg of defatted rice bran, 67kg of water, and 2.5kg of molasses into the fermentation tank, add 500g of dry yeast powder, 100g of dry powder of lactic acid bacteria, and 20g of dry powder of Bacillus subtilis, mix well, and control the temperature at 28°C. After entering the tank, the stacking height is 70cm, and the fermentation is about 48 hours at a temperature of 50°C. After the first turning, the thickness of the stacking is reduced by 50cm. After that, the turning is once a day, and the fermentation is completed in 72 hours. Add 20g of cellulase and 20g of protease for enzymolysis, and complete the enzymolysis in 24 hours. After drying at 50°C, it is crushed to make a finished product.

Embodiment 3

[0025] Add 100kg of defatted rice bran, 82kg of water, 500g of molasses, 500g of dry yeast powder, 100g of dry lactic acid bacteria powder, and 20g of dry Bacillus subtilis powder into the fermentation tank, mix well, and control the temperature at 28°C. After entering the tank, the stacking height is 70cm, and the fermentation is about 48 hours at a temperature of 50°C. After the first turning, the thickness of the stacking is reduced by 50cm. After that, the turning is once a day, and the fermentation is completed in 96 hours. Add 20g of cellulase and 20g of protease for enzymolysis, and complete the enzymolysis in 36 hours. After drying at 50°C, it is crushed to make a finished product.

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Abstract

The invention relates to a method for preparing probitics through a two-step fermentation method by using degreased rice bran as a raw material, wherein degreased rice bran is adopted as a raw material and is added with 0.5%-5.0% of molasses, water is added to adjust the moisture content of 35-45%, activated bacteria is inoculated to ferment, an enzyme preparation is added to carry out enzymolysis, and low temperature drying is performed to obtain the probitics. According to the present invention, the degreased rice bran is selected as the main raw material, the production process is simple and environmentally friendly, and the degreased rice bran has the low cost and is rich in nutrition so as to easily achieve microorganism growth and achieve the high biological activity of the product; and compared with the probitics produced from the conventional liquid fermentation, the probiotics of the present invention have the following characteristics that: after the degreased rice bran is fermented, the protein molecules are subjected to microorganism degradation to form the small peptides, the small peptides have the biological activity, and enzymolysis is performed by using cellulase and protease after the fermenting, such that the culture medium can well be degraded and becomes the nutrient substrate of the microorganisms in the animal intestinal tract, and the micro-ecology balance in the animal intestinal tract can be well maintained.

Description

technical field [0001] The invention relates to a method for preparing a prebiotic, in particular to a method for preparing a prebiotic by using defatted rice bran as a raw material through a two-step fermentation method, and belongs to the technical field of probiotics. Background technique [0002] Probiotics, also known as microecological preparations, are biological preparations produced by probiotics through a series of processes such as fermentation, processing, and drying under the guidance of microecological theory. Beneficial microorganisms, maintain or improve the micro-ecological balance in the digestive tract of animals, enhance the immune function of animals, and relieve the stress effect of the body. [0003] Prebiotics are products produced by intestinal probiotics, mainly lactic acid bacteria, yeast and bacillus, under the control of modern fermentation technology, using a combination of liquid and solid state or directly fermented on a solid medium and then ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00
Inventor 曹镜明古博华曹轩承万建华
Owner JIANGSU BAOBAO GRP
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