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Western red cedar tissue culture rapid propagation method

A technology of Thuja group and Thuja, applied in the field of Thuja tissue culture and rapid propagation, can solve the problems of large amount of cutting branches, easy aging of plants, low reproduction rate, etc., and achieve stable botanical traits, expand population, and protect mothers. strain effect

Inactive Publication Date: 2015-09-02
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Field surveys found that Thuja thuja has extremely low seed set and serious seed abortion, and the lack of seeds has constituted the main obstacle to seed propagation; cutting propagation can solve the problem of scarcity of provenance for Thuja thuja seed reproduction, but it is easily limited by the number of mother plants, and There is a lack of systematic research on cutting propagation of Thuja thuja, and there are problems such as large amount of cutting branches, low reproduction rate, and easy aging of plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Explant collection and disinfection: Select the twigs germinated in the spring of Thuja Thuja as explants, cut them into 5-6 cm stem segments, soak them in 0.3% Xinjieermin solution for 10-15 minutes, and rotate them on a magnetic stirrer for 20 minutes. Rinse with tap water for 1 to 2 hours, transfer to the ultra-clean workbench, disinfect with 75% ethanol solution for 50s, rinse with sterile water for 5 to 6 times, disinfect with 2% sodium hypochlorite solution for 10 minutes, rinse with sterile water for 5 to 6 times, Bacterial filter paper to absorb the moisture on the surface for use;

[0018] (2) Primary culture: Cut the twigs sterilized in step (1) into stem segments of about 1.5 cm, and inoculate them into the primary medium for cluster bud induction culture. The composition of the primary medium is: GD+KT1.0mg / L+NAA0.5mg / L+6-BA4.0mg / L+AC1.0g / L+sucrose 25g / L+agar 3.5g / L, pH 5.7~5.8. The culture conditions are: 12-14 hours of light per day, light intensity ...

Embodiment 2

[0023] The method and steps are the same as in Example 1, except that the composition of the primary culture medium is: GD+ KT 3.0mg / L+NAA0.8mg / L+6-BA6.0mg / L+AC1.5g / L+sucrose 30g / L+agar 5.0g / L, pH is 5.7~5.8; the composition of the subculture medium is: GD+NAA1.0mg / L+6-BA7.0mg / L+AC1.5g / L+sucrose 30g / L+ The agar is 5.0g / L, the pH is 5.7-5.8; the composition of the rooting medium is: GD+NAA1.0mg / L+IBA3.0mg / L+sucrose 30g / L+agar 5.0g / L, the pH is 5.7-5.8.

Embodiment 3

[0025] The method and steps are the same as in Example 1, the difference is: the composition of the primary culture medium is: GD+ KT2.0mg / L+NAA0.65mg / L+6-BA5.0mg / L+AC1.25g / L+sucrose 27.5g / L+agar 4.25g / L, pH 5.7~5.8; the composition of the subculture medium is: GD+NAA0.9mg / L+6-BA6.0mg / L+AC1.25g / L+sucrose 27.5 / L+agar 4.25g / L, pH 5.7~5.8; the composition of the rooting medium is: GD+NAA0.9mg / L+IBA2.0mg / L+sucrose 27.5g / L+agar 4.25g / L, pH 5.7~ 5.8.

[0026] Precautions:

[0027] 1. When selecting thuja explants, the browning degree of early spring materials is weaker than that of summer and autumn materials, and the buds in winter enter a deep dormancy state and are not easy to grow, so it is best to use early spring materials as explants;

[0028] 2. In order to prevent the effect of browning on the explants, the explants can be cultured in blank agar medium for 5-7 days, so that the phenolic substances in the tissue partially infiltrate the medium, and the incision is healed ...

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Abstract

The invention discloses a western red cedar tissue culture rapid propagation method. The western red cedar is a peculiar endangered species in China and extremely has economic value and research value, and due to the fact that western red cedars are difficult to obtain, and the number of stock plants is extremely small, sowing breeding and using of a cutting propagation method are limited, and the tissue culture rapid propagation method can be utilized for solving the problem. The method comprises the following steps of explant disinfection, primary culture, subculture, rooting culture, acclimatization and transplant. Different medium components are used in different culture stages, a reasonable acclimatization measure is taken, a western red cedar tissue culture rapid propagation technology system can be established, and the method repeatability is good. The regenerated plant obtained through the method is stable in botanical character, the month growth coefficient of test-tube plantlets reaches 4-6, the rooting percentage reaches 70 percent, the survival rate of transplanting reaches 90 percent, large-scale commercialization seedling culture production can be carried out, and the population number of the western red cedar which is the endangered species can be effectively increased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation of thuja tissue culture. Background technique [0002] Thuja sutchuenensis Franch. is an evergreen tree of the genus Thuja L. in the Cupressaceae family. The height of the tree can reach 20m, and the diameter at breast height is more than 2m. Announced as a critically endangered tree species, it is the only living plant fossil in the world and a national treasure plant unique to my country. Due to the ancient origin of this tree species and its excellent material, it has important artistic value, collection value, medicinal value and research value. In 1999, when the Chongqing Forestry Bureau organized experts to conduct a national key protected wild plant survey, wild populations of this species were found in the mountainous areas of Chengkou County and Kaixian County in Chongqing, but the number was limited, the natural re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张桂玲温四民
Owner LINYI UNIVERSITY
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