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Tissue culture rapid-propagation method for cymbidium bicolor

A technology of sclerophylla group and seeds, which is applied in the field of plant tissue culture research, can solve unseen and other problems, and achieve the effects of inhibiting browning, stable botanical traits and high reproduction coefficient.

Inactive Publication Date: 2015-12-16
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the market demand increases year by year, the large reduction of wild resources will not be able to meet people's demand for sclerophyllum. The use of tissue culture technology to produce tissue culture seedlings will be the main seedling source for large-scale cultivation of sclerophyllum. See its research report on tissue culture rapid propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. a sclerophylla tissue culture rapid propagation method, is characterized in that comprising the following steps:

[0014] (1) Sterilization of capsules: Collect eight-mature sclerophyll capsules, sterilize with 75% alcohol for 20 seconds, 0.1% mercury liter for 10 minutes, rinse with sterile water 4 to 5 times, and then sterilize with 0.1% mercury liter for 3 minutes , rinse with sterile water 4 to 5 times, and dry the surface moisture;

[0015] (2) Seed germination culture: cut the sterilized capsules under aseptic conditions, spread the seeds evenly on the seed germination medium, the composition of the seed germination medium is VW+6-BA0.3mg / L+NAA0.2mg / L+AC1.0g / L+sucrose 30g / L+agar 6g / L, pH is 5.8, the seeds turn from light yellow to green after 20-25 days of culture, and develop into protocorms after 30-35 days of culture. Orchid protocorms have strong vitality and stable genetic traits, which are extremely beneficial to rapid propagation and preservation of ge...

Embodiment 2

[0022] 1. a sclerophylla tissue culture rapid propagation method, is characterized in that comprising the following steps:

[0023] (1) Sterilization of capsules: Collect eight-mature sclerophyll capsules, sterilize with 75% alcohol for 20 seconds, 0.1% mercury liter for 10 minutes, rinse with sterile water 4 to 5 times, and then sterilize with 0.1% mercury liter for 3 minutes , rinse with sterile water 4 to 5 times, and dry the surface moisture;

[0024] (2) Seed germination culture: cut the sterilized capsules under aseptic conditions, spread the seeds evenly on the seed germination medium, the composition of the seed germination medium is VW+6-BA0.2mg / L+NAA0.1mg / L+AC1.5g / L+sucrose 30g / L+agar 6g / L, pH is 6.0, the seeds turn from light yellow to green after 20-25 days of cultivation, and develop into protocorms after 30-35 days of cultivation;

[0025] (3) Proliferation culture: Cut the protocorm into small pieces and inoculate it into the proliferation medium. The composit...

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PUM

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Abstract

The invention discloses a tissue culture rapid-propagation method for cymbidium bicolor, and belongs to the field of plant tissue culture research. The cymbidium bicolor is cymbidium epiphytes, grows on trees in a forest or bushwood and gas great appreciate and medicinal value. The method includes the steps of capsule sterilization, seed germination culture, multiplication culture, differentiation culture, rooting culture and seedling exercising and transplanting. Through the steps, tissue culture and rapid and stable propagation of cymbidium bicolor are achieved. The method is good in repeatability and high in propagation coefficient, obtained regeneration seedlings are robust and neat, botanical characters are stable, the cymbidium bicolor can be propagated within short time on a large scale, and the method has great meaning for germplasm resource protection and excellent seedling industrialized production of the cymbidium bicolor.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and in particular relates to a method for rapid propagation of sclerophyllum tissue culture. Background technique [0002] Cymbidium bicolor Lindl is an epiphytic plant of the genus Orchid, born on trees in forests or bushes, with an altitude of up to 1600 meters. It is mainly produced in Guangdong, Hainan, Guangxi, Guizhou and Yunnan from southwest to south. High ornamental and medicinal value. The leaves are strip-shaped, thick leathery, and the flowering period is March to April. The raceme has 10 to 20 flowers. The flower color is gorgeous. The whole plant can be used as medicine. Like other orchids, the seeds of sclerophyllia are usually not fully developed. It takes a long time to obtain flowering plants through seed propagation under natural conditions, and the germination rate and survival rate are extremely low, so wild sclerophyllias are very rare. In addition, due to long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张桂玲温四民
Owner LINYI UNIVERSITY
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