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Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors

An elicitor and saponin technology, applied in the field of saponin accumulation, can solve the problems of low yield, ecological environment damage, long growth cycle, etc., and achieve the effect of high saponin content

Inactive Publication Date: 2015-09-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ginsenosides, as a main active ingredient in ginseng, have low yields and have not yet been artificially synthesized
At present, ginseng mainly relies on field cultivation. However, the cultivated ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" and ecological environment damage. People's needs, people try to improve the content of ginseng secondary metabolites through various ways, such as tissue culture and reactor expansion culture, fed material culture, two-step culture, precursor feeding, induction of hairy roots, elicitor addition of
[0004] At present, the synergistic effect of two elicitors using hydrolyzed milk protein and methyl jasmonate has not been reported in the adventitious root culture of ginseng

Method used

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  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors
  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors
  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of the mother liquor of the hydrolyzed milk protein aqueous solution: the hydrolyzed milk protein was dissolved in distilled water to prepare a solution with a concentration of 1 mg / ml, which was sterilized by filtration through a 0.22 μm filter membrane.

[0020] Preparation of mother liquor of methyl jasmonate ethanol solution: Methyl jasmonate was prepared with absolute ethanol to a solution with a concentration of 1 mg / ml, and sterilized by filtration through a 0.22 μm filter membrane.

Embodiment 2

[0022] Adopt two kinds of elicitors to promote the method for saponin accumulation in Radix Ginseng Adventitious Root, comprising the following steps:

[0023] Select well-growing adventitious roots of ginseng and inoculate them into 2.5L modified 3 / 4MS liquid medium, culture them for 29 days under sterile conditions at 23±2°C, adjust the ventilation rate to 0.3vvm, and natural light conditions, and add as elicitors for implementation The hydrolyzed milk protein aqueous solution mother liquor and methyl jasmonate ethanol solution mother liquor prepared in Example 1, so that the final concentration of hydrolyzed milk protein is 98mg / L, and the final concentration of methyl jasmonate is 8mg / L, and then sterile, 23 ± 2 ℃ , adjust the ventilation rate to 0.3vvm, cultivate under natural light conditions for 2 days, harvest and dry.

[0024] Preparation of the modified 3 / 4MS liquid medium: add sucrose to the 3 / 4MS liquid medium to make the final concentration 35g / L, add indolebutyri...

Embodiment 3

[0040] Adopt two kinds of elicitors to promote the method for saponin accumulation in Radix Ginseng Adventitious Root, comprising the following steps:

[0041] Select the well-growing adventitious roots of ginseng and inoculate them into 2.5L modified 3 / 4MS liquid medium, culture them for 30 days under sterile conditions at 23±2°C, adjust the ventilation rate to 0.4vvm, and natural light conditions, and add as elicitors for implementation The hydrolyzed milk protein aqueous solution mother liquor and the methyl jasmonate ethanol solution mother liquor prepared in Example 1, so that the final concentration of the hydrolyzed milk protein is 99 mg / L, and the final concentration of methyl jasmonate is 9 mg / L, and then sterile, 23 ± 2 ° C , adjust the ventilation rate to 0.4vvm, cultivate under natural light conditions for 5 days, harvest and dry.

[0042] Preparation of the modified 3 / 4MS liquid medium: add sucrose to the 3 / 4MS liquid medium to make the final concentration 30g / L, ...

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Abstract

The invention discloses a method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors. The method comprises the following steps: picking the ginseng adventitious roots which grow well and inoculating into a modified 3 / 4 MS liquid culture medium; culturing for 28-30 days; adding a lactoalbumin hydrolysate water solution mother solution and a jasmonic acid methyl ester ethanol mother solution which are used as the inductors, wherein the final concentration of lactoalbumin hydrolysate is 98mg / L-100mg / L and the final concentration of jasmonic acid methyl ester is 8mg / L-10mg / L; and continuing to culture for 2-7 days, harvesting and drying. An experiment shows that the two inductors including lactoalbumin hydrolysate and jasmonic acid methyl ester can express the synergistic effect and do not have influences on secondary metabolism of the ginseng adventitious roots; the content of the saponin is high and evidences are provided for the large-scale production of ginsengs; and the industrial production of the ginseng saponin is realized and the day-by-day increasing requirements of people are met.

Description

technical field [0001] The invention belongs to the field of crop biotechnology, and in particular relates to a method for promoting saponin accumulation in adventitious roots of ginseng by using two elicitors. Background technique [0002] Ginseng is a perennial dicotyledonous plant of the family Araliaceae. It has the functions of enhancing immunity, preventing and treating cardiovascular diseases, protecting liver and liver, regulating blood sugar, anti-tumor, delaying aging, and enhancing learning and memory abilities. Commonly used in pharmaceuticals, health care medicines and cosmetics industries. [0003] Ginsenosides, as a class of main active ingredients in ginseng, have low yields and have not yet been artificially synthesized. At present, ginseng mainly relies on field cultivation. However, the cultivated ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" and ecological environment damage. People's needs, people try to improve the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远王娟李金鑫李静李建丽刘书杰
Owner TIANJIN UNIV
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