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Method for improving content of various steroid saponins in rhizome of paris polyphylla var.yunnanensis

A technology of steroidal saponins and Polygonum polyphylla, applied in horticultural methods, botanical equipment and methods, steroidal compounds, etc., can solve the problem of steroidal saponin content that has not yet appeared, and achieve simple and easy implementation process and simple equipment Inexpensive, less investment effect

Inactive Publication Date: 2011-07-27
WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there has been no report on the use of methyl dihydrojasmonate to increase the content of steroidal saponins in the rhizome of Prunus edulis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve methyl dihydrojasmonate with alcohol with a mass percentage concentration ≥ 75%, the amount of alcohol can completely dissolve methyl dihydrojasmonate, then dilute with water to make dihydrojasmone with a concentration of 30mg / L Methyl dihydrojasmonate solution, in the evening from July to August every year, evenly spray methyl dihydrojasmonate solution on both sides of the leaves of Dihydrojasmonic acid, and take another amount of water equal to the amount of spraying above, for untreated The front and back sides of the leaves on the plants of Dihydrojasmonate sprayed with methyl dihydrojasmonate solution were evenly sprayed on the front and back sides of the leaves, as a control. Spray once a year until its rhizomes can be used as medicine. In case of rain, re-spraying can be carried out. After the seedlings of Chonglou dianthus fell, RP-HPLC method was used to detect the content of steroidal saponins in the rhizomes. The calculated growth rate of the total...

Embodiment 2

[0022] The experiment was carried out in a method similar to that of Example 1, except that the methyl dihydrojasmonate solution with a concentration of 45 mg / L was used for spraying. The calculated growth rate of the total saponin content in the rhizomes of the current year and previous years is 15% and 38%, respectively.

Embodiment 3

[0024] The experiment was carried out in a method similar to that of Example 1, except that the methyl dihydrojasmonate solution with a concentration of 60 mg / L was used for spraying. The calculated growth rate of the total saponin content in the rhizomes of the current year and previous years is 10% and 11%, respectively.

[0025] The method of the invention has remarkable effects, and provides a simple and feasible way for increasing the content of steroidal saponins in the rhizomes of Prunella diangi and improving the quality of the rhizomes.

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PUM

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Abstract

The invention discloses a method for improving the content of various steroid saponins in the rhizome of paris polyphylla var.yunnanensis. The method comprises the following steps: the methyl dihydrojThe invention discloses a method for improving the content of various steroid saponins in the rhizome of paris polyphylla var.yunnanensis. The method comprises the following steps: the methyl dihydrojasmonate is dissolved by using alcohol with concentration being higher than or equal to 75 percent according to the mass percent, and then water is added in for dilution to obtain methyl dihydrojasmonasmonate is dissolved by using alcohol with concentration being higher than or equal to 75 percent according to the mass percent, and then water is added in for dilution to obtain methyl dihydrojasmonate solution with concentration of 30 to 60mg / L; the methyl dihydrojasmonate solution is spayed on the fully-expanded leaves of the paris polyphylla var.yunnanensis without stopping until the methyl date solution with concentration of 30 to 60mg / L; the methyl dihydrojasmonate solution is spayed on the fully-expanded leaves of the paris polyphylla var.yunnanensis without stopping until the methyl dihydrojasmonate solution on the leaves drops once a year in July or August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. The method has the advantages of simple anihydrojasmonate solution on the leaves drops once a year in July or August until the rhizoma of the paris polyphylla var.yunnanensis can be used as medicine. The method has the advantages of simple and inexpensive medicine and the instrument equipment, thereby having less investment and low cost, being easy to implement and remarkably increasing the content of the saponin; and in addition, the metd inexpensive medicine and the instrument equipment, thereby having less investment and low cost, being easy to implement and remarkably increasing the content of the saponin; and in addition, the method is non-toxic to human and animals, has no pollution to the environment and has broad application prospect.hod is non-toxic to human and animals, has no pollution to the environment and has broad application prospect.

Description

technical field [0001] The invention relates to a method for increasing the content of various steroidal saponins in the underground rhizomes of plants, in particular to a method for promoting the production of various steroidal saponins in the rhizomes of Dihydrojasmonic acid methyl ester. method for saponin content. Background technique [0002] Dianzhonglou is a perennial herbaceous plant belonging to the Trilliumaceae family. It is mainly distributed in southwest China, especially in Yunnan, Sichuan and Guizhou. Dianzhonglou is a famous and precious medicinal plant. The steroidal saponins contained in its rhizome mainly include pinanoside and diosgenin, which are its main active ingredients. It has anti-cancer, hemostatic, anti-inflammatory, analgesic, cough-relieving Asthma and immunomodulatory activities. The phenological characteristics of the growth and development of Paronymus yunnanensis are as follows: from April to June, the terminal buds of the rhizomes are un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00A01G7/06A01N37/42A01P21/00
Inventor 李家儒马青樊启龙王倩男王艳
Owner WUHAN UNIV
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