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Method for separating and preparing lithocarpus polystachyus rehd phlorizin monomer

A technology of Rhizoma polyphyllum and Rhizoma polyphylla, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc. Effect of purification efficiency and purification fold

Inactive Publication Date: 2015-08-26
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is often difficult to achieve high purity or the preparation of monomeric phlorizin with a single macroporous adsorption resin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take Lithocarpus, bake at 55°C until the water content is lower than 6%, use edible ethanol with a mass concentration of 70% as the extraction solvent, and add Edible ethanol, and assisted extraction with 50HZ ultrasound for 40min, to get the extract.

[0022]Use a 0.2 μm microfiltration membrane to perform microfiltration and clarification of the above-mentioned Lithocarpus extract at 40°C and 0.25 MPa, and when the weight of the filtrate is 80% of the weight of the extract, add 20% of the weight of the extract to the extract. The pure water was dialyzed once, and the filtrate and dialysate were collected and combined to obtain a clear solution. The microfiltration clarified liquid is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 2500Da at 30°C and 1.5Mpa, and when the filtrate weighs 80% of the clarified liquid weight, add 20% of the clarified liquid weight to the clarified liquid Dialyze once with pure water, collect filtrate and ...

Embodiment 2

[0025] Take Lithocarpus, bake at 65°C until the water content is lower than 6%, use edible ethanol with a mass concentration of 80% as the extraction solvent, and add Edible ethanol was assisted by 50HZ ultrasonic extraction for 30min, and the extract was taken.

[0026] Use a 0.2 μm microfiltration membrane to perform microfiltration and clarification of the above-mentioned Lithocarpus extract at 50°C and 0.35 MPa, and when the weight of the filtrate is 90% of the weight of the extract, add 10% of the weight of the extract to the extract. The pure water was dialyzed once, and the filtrate and dialysate were collected and combined to obtain a clear solution. The microfiltration clarified liquid is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 2500Da at 40°C and 2.0 Mpa, and when the weight of the filtrate is 90% of the weight of the clarified liquid, 10% of the weight of the clarified liquid is added to the clarified liquid. Dialyze once wi...

Embodiment 3

[0029] Take Lithocarpus, bake at 60°C until the water content is lower than 6%, use edible ethanol with a mass concentration of 75% as the extraction solvent, and add Edible ethanol was assisted by 50HZ ultrasonic extraction for 35min, and the extract was taken.

[0030] Use a 0.2 μm microfiltration membrane to perform microfiltration and clarification of the above-mentioned Lithocarpus extract at 45°C and 0.30 MPa, and when the weight of the filtrate is 85% of the weight of the extract, add 15% of the weight of the extract to the extract. The pure water was dialyzed once, and the filtrate and dialysate were collected and combined to obtain a clear solution. The microfiltration clarified liquid is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 2500Da at 35°C and 1.75 MPa, and when the weight of the filtrate is 85% of the weight of the clarified liquid, 15% of the weight of the clarified liquid is added to the clarified liquid Dialyze once wi...

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PUM

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Abstract

The invention provides a method for separating and preparing a lithocarpus polystachyus rehd phlorizin monomer. The method comprises the following steps: firstly clarifying lithocarpus polystachyus rehd extract via a microfiltration membrane; then preliminarily separating obtained clarified liquid by using an ultrafiltration membrane, and further separating and purifying obtained separated liquid by using ADS-7 macroporous adsorption resin; and finally carrying out crystallization. The method uses the widely available lithocarpus polystachyus rehd with high phlorizin content as a raw material, integrates green and safe microfiltration clarification, ultrafiltration separation, macroporous adsorption resin purification and crystallization technologies, solves the problems of high cost of phlorizin preparation using apple peels or leaves as raw materials, lack of safety of an organic solvent extraction method, low yield and low purity of a single resin separation method in the prior art; meanwhile, the lithocarpus polystachyus rehd phlorizin prepared by using the method reaches the requirements on highly-purified products or monomers; thus, high-quality raw materials are provided for the researches on phhrmalology and efficacy of the lithocarpus polystachyus rehd phlorizin and the development of functional terminal products; safe, simple, convenient, reasonable, economic and high-value application of lithocarpus polystachyus rehd resources is truly realized; and the concept of green chemical production and the strategy of benign recycling economy are fully embodied.

Description

technical field [0001] The present invention relates to a method for separating and preparing root bark glycoside monomers of A. polyaciae, especially after clarification treatment of an extract of A. polyaciae by a microfiltration membrane with a membrane pore size of 0.2 μm. A method for preliminarily separating phlorizin in clarified liquid by a filter membrane, and further separating and purifying the phlorizin monomer by using ADS-7 resin. Background technique [0002] Phlorizin is the 2'-β-D-glucoside of phloretin, which belongs to dihydrochalcone glycoside in flavonoids. Phlorizin can regulate blood sugar and blood pressure, protect the heart, and has anti-allergic, anti-inflammatory, anti-oxidation, anti-tumor and other biological activities. It is one of the hot natural products used in medicine, beauty, food, health products and other health fields at home and abroad. , and mainly derived from apple peel or leaves. However, due to the low content of phloridzin in...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 肖文军秦昱段继春丰金玉
Owner HUNAN AGRICULTURAL UNIV
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