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Rotating disk microfluidic concentration measurement device and method based on photometric detection

A concentration measurement and detection device technology, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems that the optimal optical path value is difficult to judge in advance, the concentration value of the object to be detected cannot be known in advance, and cannot be applied, so as to make up for the detection accuracy defect , easy to be portable, and reduce the effect of detection error

Active Publication Date: 2018-01-16
天裕(无锡)装备技术有限公司
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Problems solved by technology

However, in the actual detection process, the concentration value of the substance to be detected is often not known in advance, so it is difficult to judge in advance the selection of the optimal optical path value
In addition, this device is only suitable for photometric detection on a traditional scale, and is often not applicable to microfluidic photometric detection systems that require a higher optical path

Method used

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  • Rotating disk microfluidic concentration measurement device and method based on photometric detection
  • Rotating disk microfluidic concentration measurement device and method based on photometric detection
  • Rotating disk microfluidic concentration measurement device and method based on photometric detection

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Embodiment Construction

[0024] see figure 1 , is the overall structure diagram of a rotating disk microfluidic concentration measuring device based on photometric detection in the present invention. Inside a sealed darkroom 37, components such as a rotating disk microfluidic chip 1, an experimental device base 22, a photometric detection device bracket 16, and an AC servo motor 13 are arranged. Wherein, the experimental device base 22 is fixed on the bottom wall of the sealed darkroom 37, the photometric detection device support 16 is perpendicular to the experimental device base 22, and is also perpendicular to the bottom surface of the sealed darkroom 37, and the bottom of the photometric detection device support 16 is vertically welded and fixed In the center of the experimental device base 22 and the sealed dark room 37 near the left edge. The base 22 of the experimental device and the bracket 16 of the photometric detection device constitute the most basic supporting frame of the whole device....

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Abstract

The invention discloses a rotating disk microfluidic concentration measurement device and method based on photometric detection. A rotating disk microfluidic chip, a photometric detection device bracket, and an AC servo motor are arranged inside a sealed dark room, and the output shaft of the AC servo motor is vertical to the motor axis. A horizontal rotating disk microfluidic chip is installed upward and coaxially. The rotating disk microfluidic chip is equipped with an N-level test solution interpenetration zone, and each level of test solution interpenetration zone is based on the center It is an arc shape in the center of the circle, and the spring-like test solution flow channel between the adjacent two-stage test solution interpenetration zones is connected and communicated; the rotating disc microfluidic chip is used in combination with the rotating dilution positioning technology to realize the effective positioning of the dilution concentration, thereby Quickly calculate the dilution ratio, and multiply the dilution ratio by the corresponding relationship between the known optical path and the optimal concentration to generate the absorbance to deduce the precise concentration of the liquid to be tested. The process of sample injection, detection, and concentration calculation are all automated.

Description

technical field [0001] The invention relates to microfluidic photometric detection technology, in particular to a microfluidic mixed solution concentration measurement device and method based on photometric detection. Background technique [0002] Microfluidic photometric detection technology is currently the most widely applicable microfluidic detection method. However, the photometric detection cell of the microfluidic chip is often fixed, and its optical path cannot be changed arbitrarily according to the concentration of the liquid to be detected. But according to the spectrophotometric error theory, the best absorbance in the measurement A OPT is 1 / ln10, and the corresponding optimal optical path length is 0.434 αC 0 ( C 0 is the concentration of absorbate in the solution, α is the absorbance coefficient of the absorber). Therefore, only when the concentration is compatible with the detection optical path, the photometric detection noise is the smallest, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 杨宁黄余项昌华张荣标孙俊
Owner 天裕(无锡)装备技术有限公司
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