Quantitative method for free fetal DNA (deoxyribonucleic acid) proportion in maternal peripheral blood
A technology for the peripheral blood and fetus of pregnant women, applied in the field of DNA quantification, can solve the problems of low accuracy and easy deviation, and achieve the effect of improving accuracy
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[0090] The present invention introduces the DNA high-throughput sequencing method into the technology for measuring the proportion of fetal free DNA in pregnant women's free DNA, and the flow of the specific application process is further described in conjunction with specific examples.
[0091] 1. Materials
[0092] The samples provided were 4 cases of peripheral blood of pregnant women with unknown fetal cell-free DNA concentration, including 2 cases of male fetuses and 2 cases of female fetuses. DNA concentrations were compared.
[0093] High-throughput sequencing reagents are mainly composed of 3 kits, library construction kit (amplification library construction kit and ligation library construction kit); sequencing template preparation kit and sequencing kit.
[0094] 2. Method
[0095] The four samples were built and processed on the computer according to the above experimental steps to obtain sample data, and then data analysis was performed.
[0096] 1) Data pr...
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