Detection method of single nucleotide polymorphism of fshr gene in Qianbei Ma sheep

Abstract

The invention provides a method for detecting the single nucleotide polymorphism of an FSHR gene of a north Guizhou goat. The polymorphic site of the FSHR gene is detected by using a PCR-SSCP technology and a direct PCR product sequencing method to explore the relativity between the polymorphism and expression quantity of the FSHR gene and the lambing quantity, so that the basis is provided for further researching the genetic mechanism of reproductive traits of the north Guizhou goat. The base mutation from C to A is firstly found on 1246bpth of the tenth exon of the FSHR gene of the north Guizhou goat, the lambing quantity of a BB gene type is obviously different from the lambing quantity of an AA gene type and the lambing quantity of an AB gene type (P<0.05), while the difference between the AA gene type and the BB gene type is not obvious (P>0.05), namely that the lambing quantity of the BB gene type is obviously higher than the lambing quantity of the AA gene type and the lambing quantity of the AB gene type. Therefore, the mutation of the tenth exon site of the FSHR gene influences the lambing quantity of the north Guizhou goat, and the mutation site can generate remarkable influences to lambing, so that the BB gene type can be selected as an auxiliary selection basis for increasing the lambing quantity in the breeding of the north Guizhou goat.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting the single nucleotide polymorphism of the FSHR gene of Qianbei Ma sheep. Background technique [0002] Reproductive traits are one of the important economic traits of goats, and the reproductive performance is directly related to the production cost and production efficiency of goat breeding industry. Low litter size of goats is a quantitative trait with very low heritability (about 0.1), and it is difficult to improve this trait by traditional breeding techniques alone. Therefore, it is of great significance to screen and discover the main genes that affect the multi-fetal performance in some multi-fetal goat strains, and apply them to the breeding of multi-fetal strains to improve the reproductive performance of goats. Follicle stimulating hormone (FSH) is an important reproductive hormone, which binds to follicle stimulating hormone receptor (FSHR) to exert...

AI Technical Summary

Problems solved by technology

In recent years, there have been many reports on the FSHR gene of animals and humans at home and abroad, but there has been no report on the polymorphism of the FSHR gene in Qianbei Ma sheep.

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