A protein hydrophobic separation and purification membrane based on cellulose fibers

A cellulose fiber, separation and purification technology, applied in the direction of semi-permeable membrane separation, membrane technology, hydrolase, etc., can solve the problems of loss of cellulose fiber, complex membrane production process, large fluid flux, etc., and achieve short graft chains. , the preparation method is simple, the performance is good

Active Publication Date: 2017-08-25
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
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Problems solved by technology

The separation membrane obtained by this method maintains the unique porous shape of the fiber, so the fluid flux is large and the operating pressure drop is small, which is suitable for use as a separation membrane based on the non-sieving effect. complex
The other is to modify the cellulose after dissolving, which is essentially to make a membrane in the form of regenerated cellulose. The pore size of the membrane is regulated by various pore-forming technologies, rather than the porous structure naturally formed by the accumulation of fibers. Therefore, the obtained separation membrane Although it has a certain hydrophobicity, it can only be used as a separation membrane based on the sieving effect, losing the characteristic of cellulose fibers that can produce a porous morphology
[0006] It can be seen that there is currently a lack of a cellulose-based film-making technology that can not only maintain the advantages of cellulose fibers to produce a porous structure, but also conveniently endow them with hydrophobicity.

Method used

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  • A protein hydrophobic separation and purification membrane based on cellulose fibers
  • A protein hydrophobic separation and purification membrane based on cellulose fibers
  • A protein hydrophobic separation and purification membrane based on cellulose fibers

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preparation example Construction

[0024] According to the present invention, a protein hydrophobic separation and purification membrane is provided, and the preparation method of the membrane comprises the following steps:

[0025] ①Mercerizing treatment: disperse the cellulose fiber in the ethanol solution of alkali, stir and soak at room temperature for 12h~24h;

[0026] Wherein, the fiber is cellulose fiber through beating treatment, including one or more mixed fibers in wood fiber, herbaceous fiber and cotton fiber, preferably wood fiber and cotton fiber;

[0027] Wherein, the alkali includes one or more of sodium hydroxide, potassium hydroxide, sodium ethylate, preferably sodium hydroxide or potassium hydroxide;

[0028] ②Drying: remove the liquid phase material by filtration, remove ethanol from the fiber under reduced pressure at 60°C~80°C;

[0029] ③Grafting modification: place the dry fiber in a reactor and heat and dry it at 95°C~120°C for 2h~4h, then mix it with hydrophobic epoxy monomer in an iner...

Embodiment 1

[0049] Mercerizing treatment: Disperse 100g of wood fiber in a mixed solution composed of 10g of potassium hydroxide, 72g of absolute ethanol and 18g of water, stir and soak at room temperature for 24h;

[0050] Drying: After filtering, dry the fiber under reduced pressure at 70°C to remove ethanol;

[0051] Graft modification: under the protection of nitrogen, mix 50g of mercerized fibers with 10g of dry styrene oxide monomer, and react at 80°C for 24h;

[0052] Refining: carry out Soxhlet extraction and reflux for 10 h with tetrahydrofuran as solvent to remove impurities, then wash / filter with water until the pH of the filtrate is neutral. After freeze-drying, polystyrene-modified wood fibers were obtained, and the grafting rate was 12%.

[0053] Membrane production: Weigh 0.2g of polystyrene oxide modified wood fiber in the aqueous solution, stir until it is evenly dispersed, pour it into a filter cartridge with an inner diameter of 2cm, filter and press to obtain a fiber ...

Embodiment 2

[0055] Mercerizing treatment: Disperse 100g wood fiber in a mixed solution composed of 15g potassium hydroxide, 108g absolute ethanol and 27g water, stir and soak at room temperature for 24h;

[0056] Drying: After filtering, dry the fiber under reduced pressure at 80°C to remove ethanol;

[0057] Graft modification: under nitrogen protection, mix 50g of mercerized fiber with 7.5g of dry styrene oxide monomer, and react at 90°C for 24h;

[0058] Refining: carry out Soxhlet extraction and reflux for 18 hours with tetrahydrofuran as solvent to remove impurities, then wash / filter with water until the pH of the filtrate is neutral. After freeze-drying, polystyrene-modified wood fibers were obtained, and the grafting rate was 9%.

[0059] Membrane production: Weigh 0.2g of polystyrene oxide modified wood fiber in the aqueous solution, stir until it is evenly dispersed, pour it into a filter cartridge with an inner diameter of 2cm, filter and press to obtain a fiber filter cake, an...

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Abstract

The invention discloses a protein hydrophobic separation and purification membrane. The separation membrane uses cellulose fiber as a base material, undergoes mercerizing treatment, epoxy monomer ring-opening graft polymerization to obtain hydrophobic modified fibers, and disperses the modified fibers in water. The accumulated fiber layer is obtained by filtration and dried. The separation membrane has the characteristics of simple membrane making process and remarkable protein separation and purification effect.

Description

technical field [0001] The invention relates to a protein hydrophobic separation and purification membrane technology, in particular to the hydrophobic modification of cellulose fibers and its use in preparing a hydrophobic separation membrane, which is applied to protein separation and purification. Background technique [0002] The superiority of membrane separation technology is mainly manifested in that it basically operates at room temperature and has good applicability to proteins such as enzymes that are easily inactivated at high temperatures. The principle of hydrophobic chromatography protein separation membrane is to use the difference in hydrophobicity of different protein molecules to achieve the purpose of separation and purification. It only realizes the adsorption and desorption of the target protein by changing the salt concentration of the mobile phase, eliminating the need for affinity It is easier to find a suitable eluent in the chromatographic desorptio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/20B01D71/78B01D69/02B01D67/00C12N9/20
Inventor 陈晓农王蕊刘合艳陈婧泠夏宇正吴奇佳禹齐再前
Owner BEIJING UNIV OF CHEM TECH
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